Transferrin-mediated uptake of gallium-67 by EMT-6 sarcoma. I. Studies in tissue culture

Journal Article · Wed Aug 01 04:00:00 EDT 1979 · J. Nucl. Med.; (United States)

OSTI ID:6137787

Larson, S M; Rasey, J S; Allen, D R; Nelson, N J

We have studied the in vitro uptake of gallium-67 by exponentially growing EMT-6 sarcoma cells in long-term tissue culture. In this system, the addition of transferrin to the medium was required before an appreciable cellular uptake of Ga-67 occurred. The transferr in effect was complex, with an initial stimulation to a peak cell-to-medium ratio of 8 to 10:1 at low concentrations of transferrin (0.2 mg/ml), followed by a gradual decline in uptake as transferrin in the medium was increased further. EMT-6 tumor-cell uptake of Ga-67 was probably mediated by a specific cellular receptor for transferrin. Scatchard analysis of the EMT-6 cellular binding of human transferrin labeled with iodine-125 indicated a cellular receptor with affinity for transferrin of 5 x 10/sup 6/ l/mole and abundance of 500,000 receptors per cell. Over the experimental range of transferrin concentration in the medium, the observed uptake of Ga-67 was closely correlated with the degree of formaton of Ga-67-labeled transferrin and the fraction of transferrin bound to the cellular receptor (N = 69, r = 0.86, p < 0.0001).

• Research Organization: Veterans Administration Hospital, Seattle, WA
• OSTI ID: 6137787
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 20:8; ISSN JNMEA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL LOCALIZATION
CELL CULTURES
CHEMISTRY
DATA
DATA FORMS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
GALLIUM 67
GALLIUM ISOTOPES
GLOBULINS
GLOBULINS-BETA
IN VITRO
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOLATED VALUES
ISOTOPES
LABELLED COMPOUNDS
NEOPLASMS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
RECEPTORS
SARCOMAS
TRANSFERRIN
TUMOR CELLS
UPTAKE