Transferrin promotion of /sup 67/Ga and /sup 59/Fe uptake by cultured mouse myeloma cells

Journal Article · Sat Oct 01 04:00:00 EDT 1977 · Cancer Res.; (United States)

OSTI ID:5638405

Harris, A W; Sephton, R G

Radiotracer /sup 67/Ga-citrate is used as a tumor-seeking agent in clinical imaging investigations although fundamental reasons for its high uptake in certain malignant lesions remain unexplained. The mechanism by which /sup 67/Ga becomes concentrated in tumor cells has been investigated by comparing /sup 67/Ga and /sup 59/Fe uptake by cultured mouse myeloma cells with particular reference to uptake stimulation by transferrin. Concentrations of human transferrin down to 2 microgram/ml greatly stimulated cellular uptake of both tracers, whereas bovine transferrin proved relatively inactive. The rates of stimulated uptake of both tracers were similar as was their high degree of retention by cells, but their quantitative dependencies on transferrin concentration showed characteristics differences. Pretreatment of human transferrin with saturating amounts of nonradioactive Fe/sup 3 +/ canceled its ability to promote /sup 59/Fe uptake, but it had little effect on its promotion of /sup 67/Ga uptake. Further increase in the amount of added Fe/sup 3 +/ did cause a progressive depression of /sup 67/Ga uptake, but this effect probably relates to the iron distribution in the whole-cell culture system including the fetal calf serum component of cell growth medium. The results suggest that /sup 67/Ga and /sup 59/Fe reveal different aspects of the interaction of transferrin with cells.

• Research Organization: Walter and Eliza Hall Inst. of Medical Research, Melbourne, Australia
• OSTI ID: 5638405
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 37:10; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
560172* -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Animals-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL LOCALIZATION
CATTLE
CELL CULTURES
COMPARATIVE EVALUATIONS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DISEASES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GALLIUM 67
GALLIUM ISOTOPES
GLOBULINS
GLOBULINS-BETA
INTERMEDIATE MASS NUCLEI
IRON 59
IRON ISOTOPES
ISOTOPES
MAMMALS
MAN
MICE
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIONUCLIDE KINETICS
RODENTS
RUMINANTS
SCINTISCANNING
TRANSFERRIN
VERTEBRATES