A T-cell specific transcriptional enhancer element 3 prime of C sub. alpha. in the human T-cell receptor. alpha. locus

Journal Article · Fri Sep 01 04:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.17.6714· OSTI ID:7067175

Ho, Icheng; Yang, Lihsuan; Morle, G; Leiden, J M ^[1]

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1. Univ of Michigan Medical Center, Ann Arbor (USA)

A transcriptional enhancer element has been identified 4.5 kilobases 3{prime} of C{sub {alpha}} (constant region {alpha} chain) in the human T-cell receptor (TCR) {alpha}-chain locus. This enhancer is active on both a TCR V{sub {alpha}} (variable region {alpha} chain) promoter and the minimal simian virus 40 promoter in TCR {alpha}/{beta} Jurkat and EL4 cells but is inactive on a V{sub {alpha}} promoter TCR {gamma}/{delta} PEER and Molt-13 cells, clone 13 B cells, and HeLa fibroblasts. The enhancer has been localized to a 116-base-pair BstXI/Dra I restriction enzyme fragment, which lacks immunoglobulin octamer and {kappa}B enhancer motifs but does contain a consensus cAMP-response element (CRE). DNase I footprint analyses demonstrated that the minimal enhancer contains two binding sites for Jurkat nuclear proteins. One of these sites corresponds to the CRE, while the other does not correspond to a known transcriptional enhancer motif. These data support a model in which TCR {alpha} gene transcription is regulated by a unique set of cis-acting sequences and trans-acting factors, which are differentially active in cells of the TCR {alpha}/{beta} lineage. In addition, the TCR {alpha} enhancer may play a role in activating oncogene expression in T-lymphoblastoid tumors that have previously been shown to display chromosomal translocations into the human TCR {alpha} locus.

• OSTI ID: 7067175
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:17; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIGENS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
DNA
DNA SEQUENCING
DNA-ASE
ENZYME ACTIVITY
ENZYMES
ESTERASES
GENE REPRESSORS
GENETIC MAPPING
HELA CELLS
HYDROLASES
IMMUNOLOGY
LEUKOCYTES
LYMPHOCYTES
MAPPING
MATERIALS
MEMBRANE PROTEINS
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
PLASMIDS
PROTEINS
RECEPTORS
RECOMBINANT DNA
SEPARATION PROCESSES
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
THIN-LAYER CHROMATOGRAPHY
VIRUSES