The octamer motif is a B-lymphocyte-specific regulatory element of the HLA-DR. alpha. gene promoter
Journal Article
·
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
- Univ. of North Carolina, Chapel Hill (USA)
- Rockefeller Univ., New York, NY (USA)
The human class II gene, HLA-DR{alpha}, contains an octanucleotide sequence ATTTGCAT located {approx}40 base pairs upstream of the transcription initiation site. The authors have investigated the transcriptional function of the DR{alpha} octamer in human B-lymphoblastoid cells and non-B cells. Deletion and substitution mutagenesis of the octamer sequence greatly reduced the activity of the DR{alpha} promoter in both in vivo and in vitro cell-free transcription systems of B-cell origin. Conversely, these mutations did not affect promoter activity in several non-B-cell lines that express the DR{alpha} gene. Removal of octamer-binding proteins by in vivo titration with an octamer-containing competitor plasmid reduced DR{alpha} promoter activity in B-lymphoblastoid cells. These results suggest that a protein-octamer interaction, most likely involving the B-cell-specific octamer binding protein (OTF-2), is required for DR{alpha} promoter function in B-lymphoblastoid cells but not in non-B cells.
- OSTI ID:
- 7067170
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:17; ISSN PNASA; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
Similar Records
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Journal Article
·
Mon Jun 01 00:00:00 EDT 1987
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:5435190
The ubiquitous octamer-binding protein(s) is sufficient for transcription of immunoglobulin genes
Journal Article
·
Wed Feb 28 23:00:00 EST 1990
· Molecular and Cellular Biology; (USA)
·
OSTI ID:6422456
Characterization of chicken octamer-binding proteins demonstrates that POU domain-containing homeobox transcription factors have been highly conserved during vertebrate evolution
Journal Article
·
Wed Jan 31 23:00:00 EST 1990
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
·
OSTI ID:7064488
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DNA SEQUENCING
ELECTROPHORESIS
GENE REPRESSORS
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
MUTAGENESIS
NUCLEI
NUCLEOPROTEINS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
POLYMERIZATION
PRIMATES
PROTEINS
RADIOISOTOPES
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
TUMOR CELLS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DNA SEQUENCING
ELECTROPHORESIS
GENE REPRESSORS
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
MUTAGENESIS
NUCLEI
NUCLEOPROTEINS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
POLYMERIZATION
PRIMATES
PROTEINS
RADIOISOTOPES
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
TUMOR CELLS
VERTEBRATES