Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Upstream DNA sequences required for tissue-specific expression of the HLA-DR. cap alpha. gene

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ;
The authors have used in vitro deletion mutagenesis in combination with DNA transfection to search for cis-acting regulatory elements involved in the tissue-specific expression of a human class II major histocompatibility complex gene. A 140-base-pair 5' flanking fragment that contains the class II box consensus sequences and an octamer sequence (ATTTGCAT) confers tissue specificity on the promoter of the HLA-DR..cap alpha.. gene. Recombinant DNA plasmids containing this DR..cap alpha.. gene segment fused to the coding sequence of the bacterial chloramphenicol acetyltransferase gene are expressed at higher levels in human B-cell lines than in human T-cell lines. They have demonstrated that the most 5' of the class II boxes is essential for tissue-specific DR..cap alpha.. promoter function. In addition, using an electrophoretic mobility shift assay to identify DNA binding proteins, they have detected binding of nuclear proteins to DNA probes containing the class II boxes and the octamer sequence. A protein that binds to the octamer is present at higher levels in nuclear extracts of B-cell lines than in other cell lines examined. This protein may be important for the tissue-specific expression of the HLA-DR..cap alpha.. gene.
Research Organization:
Univ. of North Carolina, Chapel Hill
OSTI ID:
5435190
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:12; ISSN PNASA
Country of Publication:
United States
Language:
English

Similar Records

The octamer motif is a B-lymphocyte-specific regulatory element of the HLA-DR. alpha. gene promoter
Journal Article · Fri Sep 01 00:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA) · OSTI ID:7067170
Human X-box-binding protein 1 is required for the transcription of a subset of human class II major histocompatibility genes and forms a heterodimer with c-fos
Journal Article · Wed May 15 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States) · OSTI ID:6101245
Characterization of chicken octamer-binding proteins demonstrates that POU domain-containing homeobox transcription factors have been highly conserved during vertebrate evolution
Journal Article · Wed Jan 31 23:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA) · OSTI ID:7064488

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMALS
ATP
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DNA SEQUENCING
ELECTROPHORESIS
GENE REGULATION
GENES
HELA CELLS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
NUCLEI
NUCLEIC ACIDS
NUCLEOPROTEINS
NUCLEOTIDES
ODD-ODD NUCLEI
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
PRIMATES
PROTEINS
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
TUMOR CELLS
VERTEBRATES