Human X-box-binding protein 1 is required for the transcription of a subset of human class II major histocompatibility genes and forms a heterodimer with c-fos

Journal Article · Wed May 15 04:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.10.4309· OSTI ID:6101245

Ono, S J; Strominger, J L ^[1]; Liou, Hsiouchi; Davidon, R; Glimcher, L H ^[2]

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1. Harvard Univ., Cambridge, MA (United States)
2. Harvard Medical School, Boston, MA (United States)

A complementary DNA encoding a member of the leucine-zipper class of proteins (human X-box-binding protein, hXBP-1) that binds to the 3{prime} end of the conserved X box (X2) of the HLA-DRA major histocompatibility complex gene was recently described. Further gel-retardation analysis has demonstrated that hXBP-1 also binds to HLA-DPB X2 but not to other X2 sequences. Transient transfection of a mammalian expression vector with the hXBP-1 cDNA inserted in the antisense orientation represses the surface expression of HLA-DR and HLA-DP in Raji cells. Cotransfection of the antisense hXBP-1 vector with a HLA-DRA/chloramphenicol acetyltransferase (but not a HLA-DQB/chloramphenicol acetyltransferase) reporter plasmid decreases chloramphenicol acetyltransferase activity in Raji cells and in {gamma}-interferon-treated HeLa cells relative to cells cotransfected with a control antisense vector. Moreover, hXBP-1 is shown to form a stable heterodimer with the product of the c-fos protooncogene. These data suggest that the hXBP-1 c-fos heterodimer is critical for the transcription of a subset of the human class II major histocompatibility complex genes and that the regulatory mechanisms for the different class II genes are distinct.

• OSTI ID: 6101245
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:10; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIGENS
CARBOXYLIC ACIDS
CELL FLOW SYSTEMS
CHLORAMPHENICOL
DRUGS
ENZYMES
GENE REGULATION
GENES
HELA CELLS
HISTOCOMPATIBILITY COMPLEX
LEUCINE
ONCOGENES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PRECIPITATION
PROTEINS
SEPARATION PROCESSES
TRANSCRIPTION
TRANSFERASES