Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

B-cell-specific enhancer activity of conserved upstream elements of the class II major histocompatibility complex DQB gene

Journal Article · · Proc. Natl. Acad. Sci. USA; (United States)
;
A 95-base-pair immediate upstream sequence of the human class II major histocompatibility complex DQB gene containing the conserved X and Y elements showed enhancer activity in a transient expression assay. An enhancer test plasmid harboring the bacterial chloramphenicol acetyltransferase gene under the control of a truncated simian virus 40 enhancerless early promoter was employed. The DQB sequence inserted into this plasmid was active as an enhancer in Raji cells (human Burkitt lymphoma cells) but not active in Jurkat cells (human T-cell leukemia cells) or in HeLa cells (human cervical carcinoma cells). This cell-type specificity suggests that this enhancer activity may be involved in the tissue specificity of the DQB gene that is normally expressed only in mature B cells, macrophages, and thymic epithelial cells. Deletion analysis showed that both X and Y box sequences are essential for the full activity of the enhancer sequence and that these two sequences may function in a cooperative manner as cis-acting elements. Further deletions were used to define the 5' border of the X element. These results suggest that previously characterized protein factors that bind to X and Y include transcription factors involved in the cell-type specificity of this enhancer activity.
Research Organization:
Harvard Univ., Cambridge, MA (USA)
OSTI ID:
5822105
Journal Information:
Proc. Natl. Acad. Sci. USA; (United States), Journal Name: Proc. Natl. Acad. Sci. USA; (United States) Vol. 85:18; ISSN PNASA
Country of Publication:
United States
Language:
English

Similar Records

Human X-box-binding protein 1 is required for the transcription of a subset of human class II major histocompatibility genes and forms a heterodimer with c-fos
Journal Article · Wed May 15 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States) · OSTI ID:6101245
Conserved upstream sequences of human class II major histocompatibility genes enhance expression of class II genes in wild-type but not mutant B-cell lines
Journal Article · Mon Oct 31 23:00:00 EST 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA) · OSTI ID:5478478
Upstream DNA sequences required for tissue-specific expression of the HLA-DR. cap alpha. gene
Journal Article · Mon Jun 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States) · OSTI ID:5435190

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIGENS
AUTORADIOGRAPHY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
DNA
DNA SEQUENCING
ENZYME ACTIVITY
ENZYMES
GENE REGULATION
GENE REPRESSORS
GENES
IMMUNOLOGY
LEUKOCYTES
LYMPHOCYTES
MACROPHAGES
MATERIALS
MICROORGANISMS
MUTATIONS
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PARASITES
PHAGOCYTES
PLASMIDS
PROTEINS
SIMIAN VIRUS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
TRANSFERASES
VIRUSES