The ubiquitous octamer-binding protein(s) is sufficient for transcription of immunoglobulin genes

Journal Article · Thu Mar 01 04:00:00 EST 1990 · Molecular and Cellular Biology; (USA)

DOI:https://doi.org/10.1128/MCB.10.3.982· OSTI ID:6422456

Johnson, D G; Carayannopoulos, L; Capra, J D; Tucker, P W ^[1]; Hanke, J H ^[2]

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1. Dept. of Microbiology, Southwestern Medical Center at Dallas, Dallas, TX (US)
2. Central Research, Dept. of Molecular Genetics, Pfizer, Inc., Groton, CT (US)

All immunoglobulin genes contain a conserved octanucleotide promoter element, ATGCAAAT, which has been shown to be required for their normal B-cell-specific transcription. Proteins that bind this octamer have been purified, and cDNAs encoding octamer-binding proteins have been cloned. Some of these proteins (referred to as OTF-2) are lymphoid specific, whereas at least one other, and possibly more (referred to as OTF-1), is found ubiquitously in all cell types. The exact role of these different proteins in directing the tissue-specific expression of immunoglobulin genes is unclear. The authors have identified two human pre-B-cell lines that contain extremely low levels of OTF-2 yet still express high levels of steady-state immunoglobulin heavy-chain mRNA in vivo and efficiently transcribe an immunoglobulin gene in vitro. Addition of a highly enriched preparation of OTF-1 made from one of these pre-B cells or from HeLa cells specifically stimulated in vitro transcription of an immunoglobulin gene. Furthermore, OFT-1 appeared to have approximately the same transactivation ability as OTF-2 when normalized for binding activity. These results suggest that OTF-1, without OTF-2, is sufficient for transcription of immunoglobulin genes and that OTF-2 alone is not responsible for the B-cell-specific regulation of immunoglobulin gene expression.

• OSTI ID: 6422456
• Journal Information: Molecular and Cellular Biology; (USA), Journal Name: Molecular and Cellular Biology; (USA) Vol. 10:3; ISSN MCEBD; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL BONDS
CLONING
CONNECTIVE TISSUE CELLS
DNA HYBRIDIZATION
DNA-CLONING
GENE REGULATION
GENE REPRESSORS
GENES
GENETIC MAPPING
GLOBULINS
HYBRIDIZATION
IMMUNOGLOBULINS
LEUKOCYTES
LYMPHOCYTES
MAPPING
MATERIALS
MESSENGER-RNA
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PROTEINS
RNA
SOMATIC CELLS
SPECIFICITY
SYNTHESIS
TRANSCRIPTION