Clustering of breakpoints on chromosome 10 in acute T-cell leukemias with the t(10; 14) chromosome translocation
- Temple Univ. School of Medicine, Philadelphia, PA (USA)
The T-cell receptor (TCR){alpha}/{delta} chain locus on chromosome 14q11 is nonrandomly involved in translocations and inversions in human T-cell neoplasms. The authors have analyzed three acute T-lymphoblastic leukemia samples carrying a t(10;14)(q24;q11) chromosome translocation by means of somatic cell hybrids and molecular cloning. In all cases studied the translocation splits the TCR {delta} chain locus. Somatic cell hybrids containing the human 10q+ chromosome resulting from the translocation retain the human terminal deoxynucleotidyltransferase gene mapped at 10q23-q24 and the diversity and joining, D{sub {delta}}2-J{sub {delta}}1, regions of the TCR {delta} chain, but not the V{sub {alpha}} region (variable region of the TCR {alpha} chain), demonstrating that the split occurred within the V{sub {alpha}}-D{sub {delta}}2 region. Molecular cloning of the breakpoint junctions revealed that the TCR {delta} chain sequences involved are made from the D{sub {delta}}2 segment. The chromosome breakpoints are clustered within a region of {approx} 263 base pairs of chromosome 10. The results suggest that the translocation of the TCR {delta} chain locus to a locus on 10q, which the authors have designated TCL3, results in deregulation of this putative oncogene, leading to acute T-cell leukemia.
- OSTI ID:
- 5159994
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:11; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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CHROMOSOMAL ABERRATIONS
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HETEROCHROMOSOMES
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550401* - Genetics- Tracer Techniques