Cloning of the gene encoding the. delta. subunit of the human T-cell receptor reveals its physical organization within the. alpha. -subunit locus and its involvement in chromosome translocations in T-cell malignancy

Journal Article · Wed Jun 01 04:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.11.3933· OSTI ID:5404734

Isobe, M; Russo, G; Haluska, F G; Croce, C M ^[1]

---

1. Wistar Institute of Anatomy and Biology, Philadelphia, PA (USA)

By taking advantage of chromosomal walking techniques, the authors have obtained clones that encompass the T-cell receptor (TCR) {delta}-chain gene. They analyzed clones spanning the entire J{sub {alpha}} region extending 115 kilobases 5{prime} of the TCR {alpha}-chain constant region and have shown that the TCR {delta}-chain gene is located over 80 kilobases 5{prime} of C{sub {alpha}}. TCR {delta}-chain gene is rearranged in the {gamma}/{delta}-expressing T-cell line Peer and is deleted in {alpha}/{beta}-expressing T-cell lines. Sequence analysis of portions of this genomic region demonstrates its identity with previously described cDNA clones corresponding to the C{sub {delta}} and J{sub {delta}} segments. Furthermore, they have analyzed a t(8;14)-(q24;q11) chromosome translocation from a T-cell leukemia and have shown that the J{sub {delta}} segment is rearranged in cells deriving from this tumor and probably directly involved in the translocation. Thus, the newly clones TCR {delta} chain is implicated in the genesis of chromosome translocations in T-cell malignancies carrying cytogenetic abnormalities of band 14q11.

• OSTI ID: 5404734
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:11; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
CARCINOGENESIS
CHROMOSOMAL ABERRATIONS
CLONING
DISEASES
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GENES
GENETIC MAPPING
HEMIC DISEASES
HYBRIDIZATION
IMMUNE SYSTEM DISEASES
LEUKEMIA
LEUKEMOGENESIS
MAPPING
MEMBRANE PROTEINS
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
MUTATIONS
NEOPLASMS
NUCLEIC ACIDS
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
PATHOGENESIS
PROTEINS
RECEPTORS
STRUCTURAL CHEMICAL ANALYSIS
TUMOR CELLS