Breakpoint of an inversion of chromosome 14 in a T-cell leukemia: sequences downstream of the immunoglobulin heavy chain locus are implicated in tumorigenesis
T-cell tumors are characterized by inversions or translocations of chromosome 14. The breakpoints of these karyotypic abnormalities occur in chromosome bands 14q11 and 14q32 - the same bands in which the T-cell receptor (TCR) ..cap alpha..-chain and immunoglobulin heavy chain genes have been mapped, respectively. Patients with ataxia-telangiectasia are particularly prone to development of T-cell chronic lymphocytic leukemia with such chromosomal abnormalities. The authors describe DNA rearrangements of the TCR ..cap alpha..-chain gene in an ataxia-telangiectasia-associated leukemia containing both a normal and an inverted chromosome 14. The normal chromosome 14 has undergone a productive join of TCR ..cap alpha..-chain variable (V/sub ..cap alpha../) and joining (J/sub ..cap alpha../) gene segments. The other allele of the TCR ..cap alpha..-chain gene features a DNA rearrangement, about 50 kilobases from the TCR ..cap alpha..-chain constant (C/sub ..cap alpha../) gene, that represents the breakpoint of the chromosome 14 inversion; this breakpoint is comprised of a TCR J/sub ..cap alpha../) segment (from 14q11) fused to sequences derived from 14q32 but on the centromeric side of C/sub ..mu../. These results imply that 14q32 sequences located at an undetermined distance downstream of immunoglobulin C/sub ..mu../ locus can contribute to the development of T-cell tumors.
- Research Organization:
- Medical Research Council Laboratory of Molecular Biology, Cambridge (England)
- OSTI ID:
- 6408595
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:24
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHROMOSOMAL ABERRATIONS
GENETIC MAPPING
TUMOR CELLS
GENETICS
AUTORADIOGRAPHY
CARCINOGENESIS
DNA SEQUENCING
ELECTROPHORESIS
HETEROCHROMOSOMES
IMMUNOGLOBULINS
LEUKEMIA
PATIENTS
PHOSPHORUS 32
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
CHROMOSOMES
DAYS LIVING RADIOISOTOPES
DISEASES
GLOBULINS
HEMIC DISEASES
IMMUNE SYSTEM DISEASES
ISOTOPES
LIGHT NUCLEI
MAPPING
MUTATIONS
NEOPLASMS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PATHOGENESIS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
550401* - Genetics- Tracer Techniques