Molecular analysis of a t(14; 14) translocation in leukemic T-cells of an ataxia telangiectasia patient

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.2.602· OSTI ID:5085346

Russo, G; Isobe, M; Batuman, O; Huebner, K; Croce, C M ^[1]; Gatti, R ^[2]; Finan, J; Nowell, P C ^[3]

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1. Temple Univ. School of Medicine, Philadelphia, PA (USA)
2. Univ. of California, Los Angeles (USA)
3. Univ. of Pennsylvania School of Medicine, Philadelphia (USA)

The authors have detected and cloned two rearrangements in the T-cell receptor {alpha} locus from a clone of somatic cell hybrids carrying a t(14;14)(q11;q32) chromosomal translocation derived from an ataxia telangiectasia patient with T-cell chronic lymphocytic leukemia. The T-cell clone carrying the t(14;14) chromosomal translocation was known to be present for > 10 years before the onset of overt leukemia. One molecular rearrangement of the T-cell receptor {alpha} locus corresponded to a functional variable-joining region (V-J) joining, whereas the other derived from the breakpoint of the t(14;14)(q11;q32) translocation. Chromosomal in situ hybridization of the probe derived from the t(14;14) breakpoint localized the breakpoint region to 14q32.1, apparently the same region that is involved in another ataxia telangiectasia characteristic chromosome translocation, t(7;14)(q35;q32). The 14q32.1 breakpoint is at least 10,000 kilobase pairs (kbp) centromeric to the immunoglobulin heavy chain locus. Sequence analysis of the breakpoint indicates the involvement of a J{alpha} sequence during the translocation. Comigration of high-molecular weight DNA fragments involved with t(7;14) and t(14;14) translocations suggests the presence of a cluster of breakpoints in the 14q32.1 region, the site of a putative oncogene, TCL1.

• OSTI ID: 5085346
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:2; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
CHEMICAL ACTIVATION
CHROMOSOMAL ABERRATIONS
DISEASES
DNA
DNA SEQUENCING
ELECTROPHORESIS
GENES
GENETIC MAPPING
HEREDITARY DISEASES
KARYOTYPE
MAPPING
MUTATIONS
NUCLEIC ACIDS
ONCOGENES
ORGANIC COMPOUNDS
PATIENTS
RECOMBINANT DNA
SKIN DISEASES
STRUCTURAL CHEMICAL ANALYSIS
TELANGIECTASIS
TUMOR CELLS
VASCULAR DISEASES