Multiple nuclear proteins bind upstream sequences in the promoter region of a T-cell receptor. beta. -chain variable-region gene: evidence for tissue specificity

Journal Article · Thu Jan 01 04:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.1.232· OSTI ID:5520428

Royer, H D; Reinherz, E L

DNA-nuclear protein binding interactions were examined in the promoter region of a representative T-cell receptor Ti ..beta..-chain variable-region gene by means of electrophoretic mobility-shift and DNase I-protection analysis. Within 175 bases upstream of the transcription initiation site, four protected regions (footprints) were identified on the coding strand, at nucleotides -46 to -68 (I), -72 to -92 (II), -113 to -134 (III), and -136 to -175 (IV). Nuclear proteins of a variety of cell types produced footprints I, III, and IV and a fifth footprint. In contrast, footprint II was produced only by T-cell extracts, although nuclear extracts of a transformed B-lymphoblastoid line produced a partial footprint in this region. Furthermore, footprint analysis of the noncoding strand showed that a continuous region of protection corresponding to the entire region of footprints I and II was generated, along with a DNase I-hupersensitive site, by nuclear proteins derived from T cells but not other cell types. Footprint I has the sequence structure of many well-defined protein-DNA binding sites. Nucleotide sequences in the region of footprint II bore no homology to known sequences, whereas those in the areas of footprints III and IV were similar to motifs within immunoglobulin and other enhancers. These findings may have implications for the tissue specificity of human Ti ..beta..-chain gene transcription.

• Research Organization: Harvard Medical School, Boston, MA
• OSTI ID: 5520428
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:1; ISSN PNASA
• Country of Publication: United States
• Language: English

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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONFIGURATION INTERACTION
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DNA SEQUENCING
DNA-ASE
ELECTROPHORESIS
ENZYMES
ESTERASES
GENE REGULATION
GENE REPRESSORS
HYDROLASES
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LYMPHOCYTES
MATERIALS
MEMBRANE PROTEINS
NUCLEI
NUCLEOPROTEINS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RECEPTORS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS