Genomic footprinting; Detection of putative regulatory proteins in the promoter region of the interferon. alpha. -1 gene in normal human tissues

Journal Article · Fri Jun 01 04:00:00 EDT 1990 · Molecular and Cellular Biology; (USA)

DOI:https://doi.org/10.1128/MCB.10.6.2554· OSTI ID:6511390

Palmieri, M; Tovey, M G ^[1]

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1. Lab. of Viral Oncology, Centre National de la Recherche Scientifique, Villejuif (FR)

Dimethyl sulfate (DMS) genomic footprinting revealed the presence of putative regulatory proteins attached to specific sequences of the promoter region of the interferon (IFN) {alpha}-1 gene in normal human tissue. The pattern of protein-DNA interactions observed for the human {alpha}-1 promoter is freshly isolated human spleen cells was identical to that seen in DNA from the B-cell line Namalwa. The protein interactions involving the human IFN {alpha}-1 promoter spanned a region from positions {minus}38 to {minus}174 relative to the cap site which encompasses that part of the IFN {alpha}-1 promoter previously shown by deletion analysis to confer virus inducibility on the IFN {alpha}-1 gene. DNase I footprinting performed on isolated nuclei revealed a pattern of protein-DNA interactions for the promoter region of the IFN {alpha}-1 gene similar to that obtained with DMS footprinting performed on whole cells, with the appearance or disappearance of only a few additional protected nucleotides outside the region identified by the use of DMS. These results provide the first direct evidence for the presence of proteins bound in vivo to those parts of the IFN {alpha}-1 promoter between positions {minus}64 and {minus}109 previously shown by deletion analysis to confer virus inducibility on the IFN {alpha}-1 gene.

• OSTI ID: 6511390
• Journal Information: Molecular and Cellular Biology; (USA), Journal Name: Molecular and Cellular Biology; (USA) Vol. 10:6; ISSN MCEBD; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DNA
DNA SEQUENCING
DNA-ASE
ENZYMES
ESTERASES
GENE AMPLIFICATION
GENE REPRESSORS
GENES
GENETIC MAPPING
GROWTH FACTORS
HYDROLASES
INTERFERON
KINETICS
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MAPPING
MATERIALS
MITOGENS
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PROTEINS
REACTION KINETICS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION FACTORS