Induction of human interferon gene expression is associated with a nuclear factor that interacts with the site of the human immunodeficiency virus-enhancer

Journal Article · Thu Jun 01 04:00:00 EDT 1989 · Journal of Virology; (USA)

OSTI ID:7151907

Hiscott, J; Alper, D; Cohen, L; Leblanc, J F; Sportza, L; Wong, A; Xanthoudakis, S ^[1]

---

1. McGill Univ., Montreal, Quebec (Canada)

The relationship between transcription of alpha and beta interferon (IFN-{alpha} and IFN-{beta}) genes and the interaction of IFN promoter-binding transcription factors has been examined in monoblastoid U937 cells following priming with recombinant IFN-{alpha}2 (rIFN-{alpha}2) and Sendai virus induction. Pretreatment of U937 cells with rIFN-{alpha}2 prior to Sendai virus infection increased the mRNA levels of IFN-{alpha}1, IFN-{alpha}2, and IFN-{beta} as well as the final yield of biologically active IFN. Analysis of nuclear protein-IFN promoter DNA interactions by electrophoretic mobility-shift assays demonstrated increased factor binding to IFN-{alpha}1 and IFN-{beta} regulatory domains, although no new induction-specific complexes were identified. On the basis of competition electrophoretic mobility-shift assay results, factors interacting with the IFN-{alpha}1 and IFN-{beta} promoters appear to be distinct DNA-binding proteins. Hybrid promoter-chloramphenicol acetyltransferase fusion plasmids, containing either the IFN-{beta} regulatory element or the human immunodeficiency virus enhancer element linked to the simian virus 40 promoter, were analyzed for virus and phorbol ester inducibility in epithelial and lymphoid cells, respectively. These experiments suggest that induction of IFN gene expression may be controlled in part by transcription regulatory proteins binding to an NF-{kappa}B-like site within the IFN-{beta} promoter.

• OSTI ID: 7151907
• Journal Information: Journal of Virology; (USA), Journal Name: Journal of Virology; (USA) Vol. 63:6; ISSN 0022-538X; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHLORAMPHENICOL
CONNECTIVE TISSUE CELLS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
ENZYMES
GENE REGULATION
GENE REPRESSORS
GENES
GROWTH FACTORS
INTERFERON
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MAN
MATERIALS
MESSENGER-RNA
MITOGENS
NUCLEI
NUCLEIC ACIDS
NUCLEOPROTEINS
ODD-ODD NUCLEI
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
POLYMERIZATION
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
SOMATIC CELLS
TRANSCRIPTION
TRANSFERASES
VERTEBRATES