Postinduction represssion of the. beta. -interferon gene is mediated through two positive regulatory domains

Journal Article · Mon Oct 01 04:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.87.20.7799· OSTI ID:5475852

Whittemore, L A; Maniatis, T ^[1]

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1. Harvard Univ., Cambridge, MA (USA)

Virus induction of the human {beta}-interferon ({beta}-IFN) gene results in an increase in the rate of {beta}-IFN mRNA synthesis, followed by a rapid postinduction decrease. In this paper, the authors show that two {beta}-IFN promoter elements, positive regulatory domains I and II (PRDI and PRDII), which are required for virus induction of the {beta}-IFN gene are also required for the postinduction turnoff. Although protein synthesis is not necessary for activation, it is necessary for repression of these promoter elements. Examination of nuclear extracts from cells infected with virus reveals the presence of virus-inducible, cycloheximide-sensitive, DNA-binding activities that interact specifically with PRDI or PRDII. They propose that the postinduction repression of {beta}-IFN gene transcription involves virus inducible repressors that either bind directly to the positive regulatory elements of the {beta}-IFN promoter or inactivate the positive regulatory factors bound to PRDI and PRDII.

• OSTI ID: 5475852
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 87:20; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
CHEMICAL REACTIONS
CROSS-LINKING
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
GENE REGULATION
GENE REPRESSORS
GROWTH FACTORS
HELA CELLS
INTERFERON
LYMPHOKINES
MESSENGER-RNA
MITOGENS
NUCLEIC ACIDS
NUCLEOPROTEINS
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
POLYMERIZATION
PROTEINS
RADIATIONS
RNA
TRANSCRIPTION
ULTRAVIOLET RADIATION