Coordinate viral induction of tumor necrosis factor. alpha. and interferon. beta. in human B cells and monocytes

Journal Article · Wed Mar 01 04:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.5.1490· OSTI ID:7266909

Goldfeld, A E; Maniatis, T ^[1]

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1. Harvard Univ., Cambridge, MA (USA)

Human tumor necrosis factor {alpha} (TNF-{alpha}) gene expression can be induced primarily in cells of the monocyte/macrophage lineage by a variety of inducers, including lipopolysaccharide, phorbol esters such as phorbol 12-myristate 13-acetate, and virus or synthetic double-stranded RNA (poly(I){center dot}poly(C)). In this paper the authors show that the TNF-{alpha} gene also responds to virus and phorbol 12-myristate 13-acetate in B lymphocytes and that virus is the most potent inducer of TNF-{alpha} mRNA in both monocyte and B-cell lines. In addition, they show that viral infection coinduces the expression of TNF-{alpha} and interferon {beta} mRNA and that viral induction of both genes is blocked by the kinase inhibitor 2-aminopurine. Inhibition of protein synthesis with cycloheximide had no effect on mRNA expression of the genes in one of three cell lines tested (U937) but blocked the viral induction of both genes in another (Namalwa). Thus, the regulatory factors required for mRNA induction of both genes are present prior to the addition of virus in U937 but not in Namalwa cells. However, in a third cell line (JY), cycloheximide blocked viral induction of the interferon {beta} gene but not the TNF-{alpha} gene. Taken together, these observations suggest that viral induction of TNF-{alpha} and interferon {beta} gene expression may involve overlapping pathways with both common and distinct regulatory factors.

• OSTI ID: 7266909
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:5; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARCINOGENS
CELL CULTURES
CONNECTIVE TISSUE CELLS
CYCLOHEXIMIDE
DAYS LIVING RADIOISOTOPES
DISEASES
DNA HYBRIDIZATION
DRUGS
ESTERS
FUNGICIDES
GENE REGULATION
GROWTH FACTORS
HYBRIDIZATION
INTERFERON
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MAN
MATERIALS
MESSENGER-RNA
MITOGENS
MONOCYTES
NECROSIS
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PESTICIDES
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
POLYSACCHARIDES
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
SACCHARIDES
SOMATIC CELLS
VERTEBRATES