Autocrine secretion of tumor necrosis factor under the influence of interferon-. gamma. amplifies HLA-DR gene induction in human monocytes

Journal Article · Mon Aug 01 00:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.16.6087· OSTI ID:5641933

Arenzana-Seisdedos, F; Mogensen, S C; Vuillier, F; Fiers, W; Virelizier, J L ^[1]

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1. Institut Pasteur, Paris (France)

Recombinant interferon-{gamma} (IFN-{gamma}) induced HLA-DR gene expression in both U937 and THP-1 human monocytic cell lines, although the former was only very weakly inducible. Combination of recombinant tumor necrosis factor (TNF) and IFN-{gamma} resulted in a synergistic enhancement of DR mRNA and protein induction in both cell lines. TNF alone increased the constitutive expression of the DR gene in THP-1 cells. In the HLA class II-negative U937 cells, TNF used alone was not able to induce DR gene expression. Such a negative result was not due to a lack of TNF receptor expression in U937 cells, since TNF clearly induced HLA class I and TNF gene expression in this cell line. THP-1, but not U937, cells secreted TNF under the influence of IFN-{gamma}. Neutralization of TNF by a specific antibody decreased IFN-{gamma}-induced DR antigen expression in THP-1 cultures. These observations indicate that TNF is not able to directly induce DR gene expression, but rather amplifies ongoing expression of this gene, whether constitutive or induced by IFN-{gamma}. In the two cell lines tested, the level of DR inducibility under the influence of IFN-{gamma} used alone depended on a different inducibility of TNF secretion by IFN-{gamma}. Altogether, the observations indicate that TNF, whether exogenous or endogenously produced under the influence of IFN-{gamma}, amplifies DR gene expression in monocytes, a phenomenon that may provide to such antigen-presenting cells a selective sensitivity to the DR-inducing effects of IFN-{gamma}.

• OSTI ID: 5641933
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:16; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CELL CULTURES
DAYS LIVING RADIOISOTOPES
DNA
EMISSION SPECTROSCOPY
ESTERS
FLUORESCENCE SPECTROSCOPY
GENE REGULATION
GENES
GROWTH FACTORS
IMMUNOLOGY
INTERFERON
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LYMPHOKINES
MAMMALS
MAN
MATERIALS
MESSENGER-RNA
MITOGENS
MONOCYTES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
RNA
SPECTROSCOPY
VERTEBRATES