ACE expression in monocytes is induced by cytokines, phorbol ester and steroid

Conference · Fri Mar 15 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5212961

Lazarus, D; Lanzillo, J; Fanburg, B ^[1]

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1. New England Medical Center, Boston, MA (United States)

Angiotensin converting enzyme (ACE) levels are elevated in the serum and peripheral blood monocytes (PBM) of patients with granulomatous diseases. However, the role of ACE in (Mo) physiology and the regulation of the inflammatory response is not well understood. Since Mo can be stimulated to form giant cells using phorbol esters, glucocorticoids or certain inflammatory cytokines, the authors examined production of ACE protein by normal PBM, a Mo-like cell line, THP-1, and a macrophage-like cell line, U937 following stimulation with these agents. Using a sensitive ELISA assay, they found that in U937 cells, expression of ACE protein increased by 3.4 fold with dexamethasone, 3.7. fold with phorbol 12-myristate acetate (PMA), and 5.8 fold with the two agents combined. The cytokines IL-4 and GM-CSF substantially increased ACE expression, by 7.6 and 7.7 fold respectively, with maximal effect at 0.01 U/ml, while IFN-{gamma} and TNF-{alpha} had little effect. Similar results were found with PBM and THP-1 cells. The combination of dexamethasone and PMA also induced homotypic cluster formation in PBM, suggesting a correlation between cell adhesion and ACE production. The authors conclude that ACE expression in monocytes and macrophages is stimulated by low concentration of glucocorticoids and certain inflammatory cytokines. ACE may participate in the initiation and propagation of granulomatous inflammatory processes.

• OSTI ID: 5212961
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:5
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADRENAL HORMONES
ANIMAL CELLS
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
DEXAMETHASONE
ENZYME IMMUNOASSAY
ENZYME INDUCTION
ENZYMES
ESTERS
GENE REGULATION
GLUCOCORTICOIDS
GROWTH FACTORS
HORMONES
HYDROLASES
HYDROXY COMPOUNDS
IMMUNOASSAY
INTERFERON
KETONES
LEUKOCYTES
LYMPHOKINES
MACROPHAGES
MATERIALS
MITOGENS
MONOCYTES
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PHAGOCYTES
PHORBOL ESTERS
PREGNANES
PROTEINS
SOMATIC CELLS
STEROID HORMONES
STEROIDS