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Studies on cytolytic mechanisms of activated macrophages and monocytes

Journal Article · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6846461
;
Pulmonary alveolar macrophages (PAM) and peripheral blood monocytes (PBMo) of the miniature swine can be converted to cytolytically active cells by treating with phorbol myristic acetate (PMA) or combined stimulation with recombinant human interferon - ..gamma.. (rHuIFN-..gamma..) and lipopolysaccharide (LPS). These activated PAM and PBMo become cytolytic to various targets by producing neutral proteases. H/sub 2/O/sub 2/ and cytotoxic factors. While the PAM stimulated by PMA was most active in generating H/sub 2/O/sub 2/ as well as neutral proteases, the PBMo stimulated with PMA was able to produce only H/sub 2/O/sub 2/. The mechanism of killing by PAM seemed to vary with type of target cells: H/sub 2/O/sub 2/ was effective in killing of PRBC, SRBC, and K562, but proteases were responsible for the lysis of U937 and WEHI-164. In contrast to PMA stimulation, combined stimulation with rHuIFN-..gamma.. and LPS was very effective in generation of cytotoxic factors from PAM and PBMo. These cytotoxic factors were directly toxic to various target cells including WEHI-164, U937, K562, and CRBC, but not to autologous target such as PRBC.
Research Organization:
Univ. of Health Sciences, North Chicago, IL
OSTI ID:
6846461
Report Number(s):
CONF-8604222-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOHYDRATES
CARCINOGENS
CELL KILLING
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DOMESTIC ANIMALS
ENZYMES
ESTERS
GROWTH FACTORS
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
HYDROLASES
INTERFERON
LEUKOCYTES
LIPIDS
LIPOPOLYSACCHARIDES
LUNGS
LYMPHOKINES
MACROPHAGES
MAMMALS
MATERIALS
MINIATURE SWINE
MITOGENS
MONOCYTES
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PEPTIDE HYDROLASES
PEROXIDES
PHAGOCYTES
PHORBOL ESTERS
POLYSACCHARIDES
PROTEINS
RESPIRATORY SYSTEM
SACCHARIDES
SOMATIC CELLS
SWINE
VERTEBRATES