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Lipopolysaccharide (LPS) stimulates fresh human monocytes to lyse actinomycin D-treated WEHI-164 target cells via increased secretion of a monokine similar to tumor necrosis factor

Journal Article · · J. Immunol.; (United States)
OSTI ID:6935758
; ;
The effects of lipopolysaccharide (LPS) on tumoricidal activity of human monocytes freshly isolated from peripheral blood were studied. Actinomycin D-treated WEHI-164 cells were used as targets because they are NK insensitive and are lysed rapidly by monocytes in 6-hr /sup 51/Cr-release assays. Monocytes exhibited significant spontaneous activity without endotoxin. Monocytes either pretreated for 1 hr with LPS or assayed in the presence of LPS exhibited 100- to 1000-fold increased cytolytic activity. Cytolytic activity was heat labile and trypsin sensitive, and was recovered from Sepharose S-200 columns in a single peak with an apparent m.w. between 25,000 and 40,000. Actinomycin D or cycloheximide treatment of monocytes before the addition of LPS inhibited cytolytic monokine production. Cytolytic monokine activity was practically neutralized by specific rabbit antisera to human tumor necrosis factor (TNF). It was concluded that, although fresh human monocytes exhibit spontaneous tumoricidal activity, LPS is a potent activating agent. Its stimulatory effects depend on new transcription and translation and are mediated by enhanced secretion of a cytolytic monokine similar to TNF.
Research Organization:
Duke Univ. Medical Center, Durham, NC
OSTI ID:
6935758
Journal Information:
J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 135:6; ISSN JOIMA
Country of Publication:
United States
Language:
English

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Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACTINOMYCIN
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL CULTURES
CELL KILLING
CHEMICAL ANALYSIS
CHROMATOGRAPHY
CHROMIUM 51
CHROMIUM ISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
GEL PERMEATION CHROMATOGRAPHY
INTERMEDIATE MASS NUCLEI
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
LIPIDS
LIPOPOLYSACCHARIDES
MATERIALS
MONOCYTES
NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
POLYSACCHARIDES
QUANTITATIVE CHEMICAL ANALYSIS
RADIO-RELEASE ANALYSIS
RADIOISOTOPES
SACCHARIDES
SEPARATION PROCESSES
TRANSFERASES