Spontaneous and stimulated release of tumor necrosis factor-alpha (TNF) from blood monocytes of miners with coal workers' pneumoconiosis

Journal Article · Thu Dec 01 04:00:00 EST 1988 · Am. Rev. Respir. Dis.; (United States)

DOI:https://doi.org/10.1164/ajrccm/138.6.1589· OSTI ID:6592932

Borm, P J; Palmen, N; Engelen, J J; Buurman, W A

It is generally accepted that fibrotic lung diseases are mediated by macrophage-derived cytokines. We investigated the release of the monokine tumor necrosis factor-alpha (TNF) from blood monocytes in a group of 66 coal miners and 12 non-dust-exposed individuals. Twenty-seven miners had simple Coal Workers' Pneumoconiosis (CWP). Control miners (n = 39) were matched with respect to age, years underground, and smoking. Monocytes were assayed for TNF release, spontaneously or in response to soluble (endotoxin) or particulate (coal mine dust, silica) stimulation. TNF was measured with a TNF-specific ELISA. Monocytes of all subjects responded to stimulants by the release of TNF. Dust-exposed controls' monocytes revealed higher TNF release as compared to normal controls. The greatest discriminator between control miners and cases (CWP) was coal mine dust-induced TNF release. Interestingly, the largest difference was observed between controls and those cases with a small number of opacities (0/1, 1/0, 1/1, and 1/2), giving an odds ratio of 6.3 to find an individual with a high dust-induced TNF release in the patient group.

• Research Organization: Univ. of Limburg, Maastricht (Netherlands)
• OSTI ID: 6592932
• Journal Information: Am. Rev. Respir. Dis.; (United States), Journal Name: Am. Rev. Respir. Dis.; (United States) Vol. 138:6; ISSN ARDSB
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
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& Peat-- Health & Safety
550900 -- Pathology
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CHALCOGENIDES
COAL MINERS
COAL MINING
DISEASES
EPIDEMIOLOGY
GROWTH FACTORS
HAZARDS
HEALTH HAZARDS
LEUKOCYTES
LIPIDS
LIPOPOLYSACCHARIDES
MATERIALS
MINERALS
MINERS
MINING
MITOGENS
MONOCYTES
ORGANIC COMPOUNDS
OXIDE MINERALS
OXIDES
OXYGEN COMPOUNDS
PERSONNEL
PNEUMOCONIOSES
POLYSACCHARIDES
PROTEINS
RESPIRATORY SYSTEM DISEASES
SACCHARIDES
SECRETION
SILICA
SILICON COMPOUNDS
SILICON OXIDES