Interferon-. gamma. receptor in human monocytes is different from the one in nonhematopoietic cells
The receptor for interferon-..gamma.. (IFN-..gamma..) on peripheral blood monocytes was characterized and was compared with that of human WISH cells. /sup 125/I-IFN-..gamma.. was specifically bound to both cells; however, different binding characteristics were obtained. In the case of monocytes, Scatchard analysis gave an upward concave dependency curve, indicating either multiple binding sites or a negative cooperativity among the binding sites. In contrast, a linear Scatchard plot was obtained for the binding in WISH cells. Competition studies gave even more striking differences. Acid-treated IFN-..gamma.. (95% inactivated) effectively competed with /sup 125/I-IFN-..gamma.. for binding to the receptor on WISH cells, but not on monocytes. The significance of these differences was evaluated by analyzing the various biological activities of IFN-..gamma.. in these two cell types. IFN-..gamma.. was found to induce an antiviral state in WISH cells, but not in monocytes. Acid-treated IFN-..gamma.. was found to be almost as active as IFN-..gamma.. itself in inducing HLA-DR in WISH cells, but was almost completely inactive as an HLA-DR inducer in monocytes. It is proposed that these variations in biological activity stem from the presence of different receptors for IFN-..gamma.. in monocytes and in WISH cells. Moreover it is suggested that the immunoregulatory functions of IFN-..gamma.. in monocytes are related to the presence of a distinct IFN-..gamma.. receptor in these cells.
- Research Organization:
- Weizmann Institute of Science, Rehovot, Israel
- OSTI ID:
- 6845898
- Journal Information:
- J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 136:1; ISSN JOIMA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EMBRYONIC CELLS
GROWTH FACTORS
INTERFERON
INTERMEDIATE MASS NUCLEI
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ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
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MATERIALS
MEMBRANE PROTEINS
MITOGENS
MONOCYTES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
RADIORECEPTOR ASSAY
REACTION KINETICS
RECEPTORS
TRACER TECHNIQUES