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Lanthanide ion enhancement of interferon binding to cells

Journal Article · · Biochem. Biophys. Res. Commun.; (United States)
; ;

Pretreatment of purified (/sup 125/I)-labeled human and mouse ..beta.. interferons (IFN) with lanthanum chloride (LaCl/sub 3/) enhanced 20-30 fold the binding of the (/sup 125/I)-IFNs to human A549 and mouse L cells at 0/sup 0/C and also enhanced antiviral activity in homologous cells. Although lanthanides enhanced cross-species binding of both human and mouse (/sup 125/I)-IFNs, there was no increase in cross-species antiviral activity. Unlabeled IFN not treated with LaCl/sub 3/ did not compete with (/sup 125/I)-IFN treated with LaCl/sub 3/ did compete with LaCl/sub 3/-treated (/sup 125/I)-IFN. These results suggest that lanthanide treated IFNs do not bind to the same receptors as native IFNs.

Research Organization:
Medical College of Wisconsin, Milwaukee
OSTI ID:
5073369
Journal Information:
Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 137:1; ISSN BBRCA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CHLORIDES
CHLORINE COMPOUNDS
DATA
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
GROWTH FACTORS
HALIDES
HALOGEN COMPOUNDS
INFORMATION
INTERFERON
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LANTHANUM CHLORIDES
LANTHANUM COMPOUNDS
LYMPHOKINES
MAMMALS
MEMBRANE PROTEINS
MICE
MICROORGANISMS
MITOGENS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RADIOISOTOPES
RARE EARTH COMPOUNDS
REACTION KINETICS
RECEPTORS
RESPONSE MODIFYING FACTORS
RODENTS
TRACER TECHNIQUES
VERTEBRATES
VIRUSES