Modulation of interferon-alpha and interferon-gamma receptor expression during T-lymphocyte activation and proliferation
Freshly isolated normal human T lymphocytes constitutively expressed receptors for interferon-alpha (IFN-alpha) and IFN-gamma. Upon activation, the number of IFN-alpha receptors increased, paralleling the increases in cell size to give a nearly constant density of IFN-alpha receptors. In contrast, activation of T lymphocytes with phytohemagglutinin (PHA), concanavalin A (ConA), or phorbol myristate acetate (PMA) for 18 h decreased the specific binding of radiolabeled (Cys-Tyr-Cys) rIFN-gamma to acid-washed cells. Scatchard analysis demonstrated that the decreased binding was due to a reduction in the number of high affinity IFN-gamma receptors. Moreover, resting T lymphocytes had a single class of high-affinity IFN-gamma receptors, whereas the activated cells appeared to have both high- and lower-affinity IFN-gamma binding sites. When normalized for differences in cell size, the number of high-affinity IFN-gamma receptors per unit cell surface area was approximately twofold lower on activated than on resting T lymphocytes. At least part of the decrease was due to receptor downregulation by endogenously produced IFN-gamma, since monoclonal antibodies to IFN-gamma prevented a large portion of the PHA-induced decrease in IFN-gamma receptors.
- Research Organization:
- Program Resources, Inc., Frederick, MD
- OSTI ID:
- 6350725
- Journal Information:
- J. Interferon Res.; (United States), Vol. 6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
INTERFERON
BIOCHEMICAL REACTION KINETICS
RECEPTORS
LYMPHOCYTES
CELL PROLIFERATION
PHORBOL ESTERS
BIOLOGICAL EFFECTS
CONCANAVALIN
CPB
MONOCLONAL ANTIBODIES
PHYTOHEMAGGLUTININ
AGGLUTININS
ANIMAL CELLS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARCINOGENS
CONNECTIVE TISSUE CELLS
ESTERS
GROWTH FACTORS
HEMAGGLUTININS
KINETICS
LECTINS
LEUKOCYTES
LYMPHOKINES
MATERIALS
MEMBRANE PROTEINS
MITOGENS
MUCOPROTEINS
ORGANIC COMPOUNDS
POLYSACCHARIDES
PROTEINS
REACTION KINETICS
SACCHARIDES
SOMATIC CELLS
560300* - Chemicals Metabolism & Toxicology