Characterization of the binding of radioiodinated hybrid recombinant IFN-alpha A/D to murine and human lymphoid cell lines

Journal Article · Wed Jan 31 23:00:00 EST 1990 · Journal of Interferon Research; (USA)

DOI:https://doi.org/10.1089/jir.1990.10.55· OSTI ID:6810043

Faltynek, C R; Princler, G L; Schwabe, M; Shata, M T; Lewis, G K; Kamin-Lewis, R M ^[1]

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1. Sterling Research Group, Malvern, PA (USA)

The hybrid recombinant human interferon (IFN) rIFN-alpha A/D was radioiodinated. Specific binding of (125I)rIFN-alpha A/D was observed with both human and murine cell lines. The binding of (125I)rIFN-alpha A/D to human Daudi cells had similar characteristics to the previously described binding of (125I)rIFN-alpha A or -alpha 2. The following lines of evidence demonstrated that (125I)rIFN-alpha A/D bound with high affinity to the same receptor on murine cells as murine IFN-alpha and -beta: (i) the binding of (125I)rIFN-alpha A/D to murine LBRM cells was inhibited to a similar extent by natural murine IFN-alpha, natural murine IFN-beta, and rIFN-A/D; (ii) the Kd (approximately 2 X 10(-10) M) obtained from both competition experiments and saturation binding experiments with (125I)rIFN-alpha A/D was comparable to the previously reported Kd for the binding of natural murine IFN-alpha and -beta to other murine cell lines; (iii) the size of the cross-linked (125I)rIFN-alpha A/D receptor complex formed on murine LBRM cells was similar to the previously reported cross-linked complex formed after binding radioiodinated natural murine IFN-beta to other murine cell lines. Due to the current lack of readily available recombinant murine IFN-alpha or -beta for radiolabeling and the previously demonstrated biological activity of rIFN-alpha A/D on murine cells, (125I)rIFN-alpha A/D should prove to be a useful reagent for further studies of murine IFN receptors.

• OSTI ID: 6810043
• Journal Information: Journal of Interferon Research; (USA), Journal Name: Journal of Interferon Research; (USA) Vol. 10:1; ISSN 0197-8357; ISSN JIRED
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GROWTH FACTORS
INTERFERON
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
MICE
MITOGENS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES