Metabolism of 1,2-(1-/sup 14/C) dipalmitoyl phosphatidylcholine in the developing brain

Technical Report · Mon Jan 01 04:00:00 EST 1973

OSTI ID:7065346

Dhopeshwarkar, G A; Subramanian, C; Mead, J F

Thirteen-day-old rats were divided into two groups; one group received 1,2-(1-/sup 14/C) dipalmitoyl phosphatidylcholine and the other 1-/sup 14/C palmitic acid in the form of an intraperitoneal injection. One half of the total number of rats in each group were sacrificed 24 hours after injection and the other half were allowed to survive for 17 days after the injection. Radioactivity incorporated into brain and liver total lipids and individual polar lipid components of the brain was determined at both intervals. Phosphatidylcholine was isolated and partially deacylated with phospholipase A/sub 2/ from Crotalus Admanteus venom. The ratio of radioactivity FA-II/FA-I 24 hours after the injection was 8.3 when the tracer was radioactive phosphatidylcholine compared to only 0.7 when radioactive palmitate was injected. From this different labeling ratio and also different pattern of labeling of the polar lipid components it was concluded that the radioactive phosphatidylcholine was not completely deacylated before being taken up directly into the brain. Possibilities are discussed to show that the observed radioactive ratio could result from direct uptake of intact phosphatidylcholine, with little or no restriction from the blood brain barrier system, followed by partial degradation by phospholipase A/sub 1/ in the brain itself.

• Research Organization: California Univ., Los Angeles (USA). Lab. of Nuclear Medicine and Radiation Biology; California Univ., Los Angeles (USA). School of Medicine
• DOE Contract Number: AC03-76SF00012
• OSTI ID: 7065346
• Report Number(s): UCLA-12-918
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BRAIN
CARBON 14
CARBON ISOTOPES
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
CHEMICAL REACTIONS
DATA
DECOMPOSITION
ESTERS
EVEN-EVEN NUCLEI
EXPERIMENTAL DATA
HEXADECANOIC ACID
INFORMATION
INJECTION
INTAKE
INTRAPERITONEAL INJECTION
ISOTOPES
LIGHT NUCLEI
LIPIDS
MAMMALS
METABOLISM
MONOCARBOXYLIC ACIDS
NERVOUS SYSTEM
NUCLEI
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
RADIOISOTOPES
RADIONUCLIDE KINETICS
RATS
RODENTS
UPTAKE
VERTEBRATES
YEARS LIVING RADIOISOTOPES