Synthesis of the dipalmitoyl species of diacyl glycerophosphocholine by rabbit alveolar macrophages

Journal Article · Sat Feb 01 04:00:00 EST 1986 · Lipids; (United States)

DOI:https://doi.org/10.1007/BF02534438· OSTI ID:5811200

Nakagawa, Y; Waku, K

The distribution of radioactivity among the molecular species of diacyl glycerophosphocholine of rabbit alveolar macrophages was determined after incubation with (/sup 3/H)glycerol and 1-(/sup 14/C)palmitoyl-sn-glycero-3-phosphocholine. The highest percentage of radioactivity of (/sup 3/H)glycerol was found in the dipalmitoyl species (35% of the total) followed by the 1-palmitoyl-2-linoleoyl (23.6%) and 1-stearoyl-2-linoleoyl plus 1-palmitoyl-2-oleoyl species (19.7%) during the first 30 min incubation. The radioactivity of the dipalmitoyl species reached a maximum at 120 min incubation and decreased thereafter, although the radioactivities of other molecular species still increased. In contrast to the (/sup 3/H)glycerol labeling, only 4% of the total radioactivity in diacyl glycerophosphocholine derived from 1-(/sup 14/C)palmitoyl glycerophosphocholine was found in the dipalmitoyl species; 80% of the radioactivity was located in the 1-palmitoyl-2-arachidonoyl species at 10 min incubation. The present results indicate that the dipalmitoyl species of diacyl glycerophosphocholine are synthesized predominantly via a de novo pathway and not the deacylation-reacylation pathway in rabbit alveolar macrophages.

• Research Organization: Teikyo Univ., Kanagawa, Japan
• OSTI ID: 5811200
• Journal Information: Lipids; (United States), Journal Name: Lipids; (United States) Vol. 2; ISSN LPDSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
BODY
CARBON 14 COMPOUNDS
CHOLINE
CONNECTIVE TISSUE CELLS
DISTRIBUTION
DRUGS
GLYCEROL
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
LUNGS
MACROPHAGES
MAMMALS
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTES
QUATERNARY COMPOUNDS
RABBITS
RESPIRATORY SYSTEM
SOMATIC CELLS
SYNTHESIS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES