Reutilization of surfactant phosphatidylcholine by isolated adult rat type II cells in culture

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5271409

Moxley, M A; Longmore, W J

The reutilization of surfactant phospholipids by the mammalian lung has been demonstrated in vivo by other laboratories. The authors have reported the uptake of native surfactant labeled with radioisotopes or fluorescent fatty acids previously. This work has been extended to determine if surfactant lipids are utilized intact or metabolized and resynthesized. Adult rat type II cells were prepared by trypsin treatment and purified by albumin gradient centrifugation and differential adherence. After 22 hrs in culture the cells were incubated in serum-free medium containing 3% bovine serum albumin, rat lung surfactant (20 ..mu..M phosphatidylcholine), 1,2(1-/sup 14/C)dipalmitoyl phosphatidylcholine and (/sup 3/H-methyl-choline)dipalmitoyl phosphatidylcholine. After a 90 min incubation the cells were scraped from the culture dishes, disrupted by homogenization and lamellar bodies isolated from other cellular fractions. The ratio of the labeled lipids in the medium was compared to that in the lamellar body fractions and in the combined residual fractions. These experiments showed that the ratio of the lamellar body label to the medium label is 0.87 and that for the combined residual fractions the ratio is 1.05 suggesting that the phosphatidylcholine is taken up and reutilized as an intact molecule by the type II cells.

• Research Organization: St. Louis Univ., MO
• OSTI ID: 5271409
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMALS
BODY
CARBON 14 COMPOUNDS
CATTLE
CELL CULTURES
CENTRIFUGATION
DOMESTIC ANIMALS
ESTERS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
LUNGS
MAMMALS
METABOLISM
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
PROTEINS
RATS
RESPIRATORY SYSTEM
RODENTS
RUMINANTS
SEPARATION PROCESSES
SURFACTANTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES