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Uptake and degradation of natural surfactant by isolated rat granular pneumocytes

Journal Article · · American Journal of Physiology; (USA)
OSTI ID:7191825
; ;  [1]
  1. Univ. of Pennsylvania, Philadelphia (USA)
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It has been previously shown that isolated granular pneumocytes internalize and degrade dipalmitoylphosphatidylcholine (DPPC) in synthetic lipid vesicles and reutilize degradation products for phosphatidylcholine (PC) synthesis. In this study, the authors evaluated the uptake and degradation of radiolabeled natural surfactant (NS) isolated from lung lavage after perfusing isolated rat lungs with ({sup 3}H)choline. Uptake of NS by isolated granular pneumocytes was increased approximately fourfold compared with synthetic liposomes, suggesting that physical form or a component (e.g., a protein) of NS plays a role in phospholipid uptake by these cells. Uptake was significantly decreased by metabolic inhibitors, indicating an energy requirement for this process. After 2-h incubation, the pattern of radioactivity in cells compared with NS showed a significant decrease in PC and DSPC and increase in free choline, choline phosphate, and CDP-choline. This pattern of metabolism indicates degradation of PC and metabolic reutilization of products. These studies support the hypothesis that alveolar phospholipids are accumulated and reutilized by granular pneumocytes for surfactant synthesis.
OSTI ID:
7191825
Journal Information:
American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 253:6; ISSN 0002-9513; ISSN AJPHA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
BIODEGRADATION
CHEMICAL REACTIONS
CHOLINE
DECOMPOSITION
DISTRIBUTION
DRUGS
ENZYME INHIBITORS
ESTERS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
LIPIDS
LIPOTROPIC FACTORS
MAMMALS
METABOLISM
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
QUATERNARY COMPOUNDS
RATS
RESPIRATORY TRACT CELLS
RODENTS
SURFACTANTS
TISSUE DISTRIBUTION
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES