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Early events leading to erythroid differentiation in mouse Friend cells revealed by cell fusion experiments

Journal Article · · Cancer Res.; (United States)
OSTI ID:7054844
; ;

Cell fusion with two genetically marked Friend (murine erythroleukemia) cells has made it possible to characterize the very early events leading to erythroid differentiation, particularly the nature of reactions initiated by inducers such as dimethyl sulfoxide. We have found that brief exposure of Friend cells to dimethyl sulfoxide (as well as butyric acid or hexamethylene-bisacetamide) induces an early cellular activity required for erythroid differentiation which is detected only by fusion with ultraviolet-irradiated cells. The induction process of this activity consists of at least two distinct stages. In the first stage, the reaction proceeds without supply of metabolites from the medium and exhibits sensitivity to tumor promoters. The second stage is tightly coupled to cellular metabolic activity, notably protein synthesis. Under normal conditions, the induced activity is short-lived, suggesting turnover of the molecules responsible for this activity. There appears to be a signal produced following dimethyl sulfoxide pulse which acts as an inducer for this activity. The signal remains active for as long as 40 hr when protein synthesis is blocked.

Research Organization:
Institute of Applied Microbiology, University of Tokyo, Japan
OSTI ID:
7054844
Journal Information:
Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 44:5; ISSN CNREA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BONE MARROW CELLS
CELL DIFFERENTIATION
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
ENZYMES
GLYCOSYL TRANSFERASES
HYBRIDOMAS
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PENTOSYL TRANSFERASES
RADIATION EFFECTS
RADIATIONS
SOMATIC CELLS
SULFOXIDES
TRANSFERASES
ULTRAVIOLET RADIATION