Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Dimethyl sulfoxide-inducible cytoplasmic factor involved in erythroid differentiation in mouse erythroleukemia (Friend) cells

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
;

A previous report described an intracellular factor (differentiation-inducing factor I, or DIF-I) that seem to play a role in erythroid differentiation in mouse erythroleukemia (MEL) cells. The authors have detected another erythroid-inducing factor in cell-free extracts from dimethyl sulfoxide- or hexamethylenebis(acetamide)-treated MEL cells, which acts synergistically with DIF-I. The partially purified factor (termed DIF-II) triggered erythroid differentiation when introduced into undifferentiated MEL cells that had been potentiated by the induction of DIF-I. The activity in the extracts appeared in an inducible manner after addition of dimethyl sulfoxide or hexamethylenebis(acetamide), reached a maximum at 6 hr, and then rapidly decreased. The induction was inhibited by phorbol 12-myristate 13-acetate and also by cycloheximide. No induction was observed in a mutant MEL cell line defective in erythroid differentiation. These characteristics are consistent with the supposition that DIF-II is one of the putative dimethyl sulfoxide-inducible factors detected in previously reported cell-fusion and cytoplast-fusion experiments. The role of DIF-II in MEL-cell differentiation and in vitro differentiation in general is discussed.

Research Organization:
Univ. of Tokyo (Japan)
OSTI ID:
6997953
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:18; ISSN PNASA
Country of Publication:
United States
Language:
English

Similar Records

Early events leading to erythroid differentiation in mouse Friend cells revealed by cell fusion experiments
Journal Article · Tue May 01 00:00:00 EDT 1984 · Cancer Res.; (United States) · OSTI ID:7054844
Effects of trichostatins on differentiation of murine erythroleukemia cells
Journal Article · Wed Jul 15 00:00:00 EDT 1987 · Cancer Res.; (United States) · OSTI ID:6274290
Induction and inhibition of friend erythroleukemia cell differentiation by pyrimidine analogs: Analysis of the requirement for intracellular accumulation and incorporation into DNA
Journal Article · Sun Aug 01 00:00:00 EDT 1982 · Mol. Cell. Biol.; (United States) · OSTI ID:5891872

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BLOOD FORMATION
CARCINOGENS
CELL DIFFERENTIATION
CHEMISTRY
CYCLOHEXIMIDE
DMSO
DRUGS
ELECTROMAGNETIC RADIATION
ERYTHROPOIESIS
ESTERS
FUNGICIDES
MAMMALS
MICE
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PESTICIDES
PHORBOL ESTERS
RADIATION EFFECTS
RADIATIONS
RODENTS
SULFOXIDES
TUMOR CELLS
ULTRAVIOLET RADIATION
VERTEBRATES