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Homologous globin cell-free transcription system with comparison of heterologous factors

Journal Article · · Mol. Cell. Biol.; (United States)
DOI:https://doi.org/10.1128/MCB.4.1.17· OSTI ID:5809890
; ; ; ;

Mouse erythroleukemia (MEL) cells provide a useful model system to examine the regulation of globin gene expression. MEL cells ordinarily do not express globin genes, but in the presence of inducers, such as dimethyl sulfoxide or hexamethylene bisacetamide, they mimic erythroid differentiation. The authors have developed a cell-free transcription system from uninduced MEL cells to determine the requirements for mRNA synthesis. The MEL system directs accurate transcription of adenovirus type 2 major late DNA and mouse ..beta../sup maj/-globin with an efficiency comparable to those of HeLa and KB cell extracts. The authors have isolated three active fractions from both MEL and HeLa cell extracts which are required for accurate transcription and have shown that equivalent fractions from MEL and HeLa cell extracts are interchangeable. Their findings suggest that the components required for initiation of transcription are similar in different cell types, at least to the extent that they can be assayed in these in vitro systems.

Research Organization:
Lab. of Molecular Hematology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20205
OSTI ID:
5809890
Journal Information:
Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 4:1; ISSN MCEBD
Country of Publication:
United States
Language:
English

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Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ADENOVIRUS
AMIDES
ANIMALS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BIOLOGICAL MODELS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENESIS
CELL CULTURES
CELL DIFFERENTIATION
DISEASES
DNA
ERYTHROCYTES
FUNCTIONS
GENE REGULATION
GLOBIN
HELA CELLS
HEMIC DISEASES
IN VITRO
LEUKEMIA
MAMMALS
MATERIALS
MESSENGER-RNA
MICROORGANISMS
NEOPLASMS
NUCLEIC ACIDS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PATHOGENESIS
PHENOTYPE
PROTEINS
RATS
RECOMBINANT DNA
RNA
RODENTS
TISSUE EXTRACTS
TRANSCRIPTION
VERTEBRATES
VIRUSES