Triglyceride kinetics, tissue lipoprotein lipase, and liver lipogenesis in septic rats

Journal Article · Sun Apr 01 04:00:00 EDT 1990 · American Journal of Physiology; (USA)

OSTI ID:7043928

Lanza-Jacoby, S; Tabares, A ^[1]

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1. Jefferson Medical College, Philadelphia, PA (USA)

The mechanism for the development of hypertriglyceridemia during gram-negative sepsis was studied by examining liver production and clearance of very-low-density lipoprotein (VLDL) triglyceride (TG). To assess liver output and peripheral clearance the kinetics of VLDL-TG were determined by a constant iv infusion of (2-3H)glycerol-labeled VLDL. Clearance of VLDL-TG was also evaluated by measuring activities of lipoprotein lipase (LPL) in heart, soleus muscle, and adipose tissue from fasted control, fasted E. coli-treated, fed control, and fed E. coli-treated rats. Lewis inbred rats, 275-300 g, were made septic with 8 x 10(7) live E. coli colonies per 100 g body wt. Twenty-four hours after E. coli injection, serum TG, free fatty acids (FFA), and cholesterol of fasted E. coli-treated rats were elevated by 170, 76, and 16%, respectively. The elevation of serum TG may be attributed to the 67% decrease in clearance rate of VLDL-TG in fasted E. coli-treated rats compared with their fasted controls. The suppressed activities of LPL in adipose tissue, skeletal muscle, and heart were consistent with reduced clearance of TG. Secretion of VLDL-TG declined by 31% in livers of fasted E. coli-treated rats, which was accompanied by a twofold increase in the composition of liver TG. Rates of in vivo TG synthesis in livers of the fasted E. coli-treated rats were twofold higher than in those of fasted control rats. Decreased rate of TG appearance along with the increase in liver synthesis of TG contributed to the elevation of liver lipids in the fasted E. coli-treated rats.

• OSTI ID: 7043928
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 258; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ALDEHYDES
ANIMAL TISSUES
ANIMALS
BACTERIA
BACTERIAL DISEASES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARBOXYLESTERASES
CARDIOVASCULAR SYSTEM
CONNECTIVE TISSUE
DIGESTIVE SYSTEM
DISEASES
ENZYMES
ESCHERICHIA COLI
ESTERASES
ESTERS
FASTING
GLANDS
GLUCAGON
GLUCOSE
HEART
HEXOSES
HORMONES
HYDROGEN COMPOUNDS
HYDROLASES
INFECTIOUS DISEASES
INSULIN
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
KINETICS
LIPASES
LIPIDS
LIPOPROTEINS
LIVER
MAMMALS
MICROORGANISMS
MONOSACCHARIDES
MUSCLES
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PEPTIDE HORMONES
PEPTIDES
POLYPEPTIDES
PROTEINS
RATS
REACTION KINETICS
RODENTS
SACCHARIDES
SYNTHESIS
TISSUES
TRACER TECHNIQUES
TRIGLYCERIDES
TRITIUM COMPOUNDS
VERTEBRATES