Triacylglycerol kinetics in endotoxic rats with suppressed lipoprotein lipase activity

Journal Article · Wed Jul 01 04:00:00 EDT 1987 · Am. J. Physiol.; (United States)

OSTI ID:6244590

Bagby, G J; Corll, C B; Martinez, R R

Hypertriglyceridemia observed in animals after bacterial endotoxin administration and some forms of sepsis can result from increased hepatic triacylglycerol (TG) output or decreased TG clearance by extrahepatic tissues. To differentiate between these two possibilities, TG and free fatty acid (FFA) kinetics were determined in control and endotoxin-injected rats 18 h after treatment. Plasma TG and FFA kinetics were assessed by a constant intravenous infusion with (9,10-/sup 3/H)palmitate-labeled very low-density lipoprotein and (1-/sup 14/C)palmitate bound to albumin, respectively. In addition, lipoprotein lipase (LPL) activity was determined in heart, skeletal muscle, and adipose tissue as well as in postheparin plasma of functionally hepatectomized, adrenalectomized, and gonadectomized rats. Plasma FFA acid concentrations were slightly increased in endotoxin-treated rats but their turnover did not differ from control. Endotoxin-treated rats had a threefold increase in plasma TG concentrations and decreased heart, skeletal muscle, and post-heparin plasma LPL activity. Plasma TG turnover was decreased, indicating that hypertriglyceridemia was not due to an increased TG output by the liver. Instead, the endotoxin-induced increase in plasma TG concentration was consequence of the 80% reduction in TG metabolic clearance rate. Thus, suppression of LPL activity in endotoxic animals impairs TG clearance resulting in hypertriglyceridemia. Furthermore, endotoxin administration reduced the delivery of TG-FFA to extrahepatic tissues because hepatic synthesis and secretion of TG from plasma FFA was decreased and LPL activity was suppressed.

• Research Organization: Louisiana State Univ. Medical Center, New Orleans (USA)
• OSTI ID: 6244590
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 253:1; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ALCOHOLS
ANIMAL TISSUES
BIOCHEMICAL REACTION KINETICS
BODY
CARBON 14 COMPOUNDS
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CONNECTIVE TISSUE
DIGESTIVE SYSTEM
DISEASES
ENZYME ACTIVITY
ENZYMES
ESTERASES
ESTERS
GLANDS
GLYCEROL
HEART
HEXADECANOIC ACID
HYDROLASES
HYDROXY COMPOUNDS
INFECTIOUS DISEASES
KINETICS
LABELLED COMPOUNDS
LIPASE
LIPIDS
LIPOPROTEINS
LIVER
METABOLIC DISEASES
MONOCARBOXYLIC ACIDS
MUSCLES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
REACTION KINETICS
TISSUES
TRIGLYCERIDES
TRITIUM COMPOUNDS