Lipoprotein lipase activity and chylomicron clearance in rats fed a high fat diet

Journal Article · Tue Nov 01 04:00:00 EST 1988 · J. Nutr.; (United States)

OSTI ID:6515280

Brown, C M; Layman, D K

The relationships of tissue and plasma lipoprotein lipase (LPL) activities to tissue uptake and plasma clearance of UC-labeled chylomicron-triglyceride ( UC-CM-TG) were studied in female rats fed isoenergetic and isonitrogenous control (12% kJ from fat) or high fat diets (72% kJ from fat) for 8 wk. Animals fed the high-fat diet had higher levels of fasting plasma triglycerides and lower LPL activities in heart, renal adipose tissue and post-heparin plasma. Changes in LPL activities of skeletal muscles varied among muscles with higher values in the soleus and plantaris (32-61%) and no differences in the gastrocnemius. The lower LPL activity in renal adipose tissue was associated with lower uptake of fatty acids from UC-CM-TG by adipose. Fatty-acid uptake from labeled TG was not associated with tissue LPL activity in other tissues. Clearance of UC-CM-TG from plasma and the half-lives of UC-CM-TG were similar in both dietary groups. These data indicate that tissue and plasma LPL activities are not a direct index of uptake of fatty acids by tissues or clearance of chylomicron triglycerides.

• Research Organization: Univ. of Illinois, Urbana (USA)
• OSTI ID: 6515280
• Journal Information: J. Nutr.; (United States), Journal Name: J. Nutr.; (United States) Vol. 118:11; ISSN JONUA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL HALF-LIFE
BLOOD-PLASMA CLEARANCE
CARBON 14 COMPOUNDS
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CHYLOMICRONS
CLEARANCE
DIET
DISTRIBUTION
ENZYME ACTIVITY
ENZYMES
ESTERASES
ESTERS
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPASE
LIPIDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
RATS
RODENTS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TRIGLYCERIDES
VERTEBRATES