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Functional analysis of the individual enhancer core sequences of polyomavirus: Cell-specific uncoupling of DNA replication from transcription

Journal Article · · Mol. Cell. Biol.; (United States)
DOI:https://doi.org/10.1128/MCB.8.5.1993· OSTI ID:7027022
;
Polyomavirus (Py) enhancer core elements were compared for their ability to activate Py early transcription and DNA replication in mouse 3T6 cells, lymphoid cell lines, and undifferentiated embryonal carcinoma cells. By examining the pattern of genetic change in a number of cell-specific Py variants, the authors identified subenhancer sequences that may be functionally important for virus replication. Four such distinct enhancer consensus sequences were synthesized and designated as the A core (homologous with adenovirus 5 E1A enhancer), B core (homologous to the simian virus 40 A enhancer core), C core (containing an inverted repeat within the Py B enhancer), and BPV core (homologous to the bovine papillomavirus enhancer). When used to replace the complete Py B enhancer, single copies of all but the BPV element were able to fully activate Py DNA replication after transfection, but this activation was usually cell type specific. In the PCC4 embryonal carcinoma cells, only the A-core sequence was able to activate transcription and DNA replication. The BPV core sequence containing the Py F441 point change was unable to activate DNA replication in the F9 embryonal carcinoma or any other cell line. No single insertion element was dominant nor did these elements display the wild-type enhancer pattern of cell-specific activation of DNA replication. In addition, differential effects were often observed on the activation of transcription versus DNA replication.
Research Organization:
Dept. of Molecular Biology and Biochemistry, Univ. of California at Irvine, Irvine, CA (US)
OSTI ID:
7027022
Journal Information:
Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:5; ISSN MCEBD
Country of Publication:
United States
Language:
English

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Related Subjects

550400* -- Genetics
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
BODY
COMPARATIVE EVALUATIONS
DNA REPLICATION
EMBRYONIC CELLS
FUNCTIONS
GENE AMPLIFICATION
GENES
KINETICS
MAMMALS
MICE
MICROORGANISMS
MOLECULAR BIOLOGY
MORPHOLOGY
MUTATIONS
NUCLEIC ACID REPLICATION
ONCOGENES
ONCOGENIC VIRUSES
PARASITES
POLYOMA VIRUS
REACTION KINETICS
RODENTS
SIMIAN VIRUS
SPECIFICITY
STRUCTURE-ACTIVITY RELATIONSHIPS
TISSUES
TRANSCRIPTION
VERTEBRATES
VIRUSES