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Two overlapping sequence motifs within the polyomavirus enhancer are independently the targets of stimulation by both the tumor promoter 12-O-tetradecanoylphorbol-3-acetate and the Ha-ras oncogene

Journal Article · · J. Virol.; (United States)
OSTI ID:5814845
; ;
A tumor-promoting phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), strongly stimulates the activity of polyomavirus enhancer in a human erythroleukemia cell line, K562. The target of stimulation was the previously defined A element (from nucleotides 5107 to 5130) of the enhancer. The authors found that within the A element, two partly overlapping sequence motifs (one from nucleotides 5107 to 5117, the other from nucleotides 5113 to 5121) were independently the targets of TPA stimulation. The former is homologous to the enhancer core sequence of the adenovirus type 5 E1A gene, and the latter shares the consensus AP-1-binding site. In addition, transiently expressed Ha-ras oncogene also stimulated these two subelements in K562 cells, as they reported for NIH 3T3 cells previously.
Research Organization:
Kyoto Univ. (Japan)
OSTI ID:
5814845
Journal Information:
J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 63:3; ISSN JOVIA
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CARCINOGENS
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
ESTERS
GENE REGULATION
GENES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ONCOGENES
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
POLYOMA VIRUS
PRIMATES
PROMOTERS
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
TUMOR CELLS
TUMOR PROMOTERS
VERTEBRATES
VIRUSES