Two overlapping sequence motifs within the polyomavirus enhancer are independently the targets of stimulation by both the tumor promoter 12-O-tetradecanoylphorbol-3-acetate and the Ha-ras oncogene
A tumor-promoting phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), strongly stimulates the activity of polyomavirus enhancer in a human erythroleukemia cell line, K562. The target of stimulation was the previously defined A element (from nucleotides 5107 to 5130) of the enhancer. The authors found that within the A element, two partly overlapping sequence motifs (one from nucleotides 5107 to 5117, the other from nucleotides 5113 to 5121) were independently the targets of TPA stimulation. The former is homologous to the enhancer core sequence of the adenovirus type 5 E1A gene, and the latter shares the consensus AP-1-binding site. In addition, transiently expressed Ha-ras oncogene also stimulated these two subelements in K562 cells, as they reported for NIH 3T3 cells previously.
- Research Organization:
- Kyoto Univ. (Japan)
- OSTI ID:
- 5814845
- Journal Information:
- J. Virol.; (United States), Vol. 63:3
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ONCOGENES
BIOLOGICAL EFFECTS
PHORBOL ESTERS
POLYOMA VIRUS
DNA SEQUENCING
DNA
GENE REGULATION
MAN
PHOSPHORUS 32
PLASMIDS
TUMOR CELLS
TUMOR PROMOTERS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARCINOGENS
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
ESTERS
GENES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS ISOTOPES
PRIMATES
PROMOTERS
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
VIRUSES
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