Loss of responsiveness of an AP1-related factor, PEBP1, to 12-O-tetradecanoylphorbol-13-acetate after transformation of NIH 3T3 cells by the Ha-ras oncogene
Journal Article
·
· Journal of Virology; (USA)
OSTI ID:7066856
- Kyoto Univ. (Japan)
The function of the A element (nucleotides 5107 to 5130) of the polyomavirus enhancer is augmented in NIH 3T3 cells by a tumor-promoting phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA). One of its targets is an AP1 consensus sequence motif recognized by a nuclear factor, PEBP1. In Ha-ras-transformed NIH 3T3 cells, however, A element function was not enhanced by TPA treatment, and at the same time PEBP1 was not detected in the nuclear extract by a mobility shift assay. PEBP1 was not detected in either the extract from NIH 3T3 cells treated in vivo with a protein kinase inhibitor, staurosporine, or the extract from NIH 3T3 cells after treatment in vitro with phosphatase. These results suggest that PEBP1 is required to be properly phosphorylated for DNA binding and that it is underphosphorylated, possibly due to the downregulation of protein kinase C in Ha-ras-transformed cells. In addition, it was observed that PEBP2, which bound to the A element adjacent to PEBP1, was converted to apparently related PEBP3 when conditions favored underphosphorylation.
- OSTI ID:
- 7066856
- Journal Information:
- Journal of Virology; (USA), Journal Name: Journal of Virology; (USA) Vol. 63:9; ISSN 0022-538X; ISSN JOVIA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Two overlapping sequence motifs within the polyomavirus enhancer are independently the targets of stimulation by both the tumor promoter 12-O-tetradecanoylphorbol-3-acetate and the Ha-ras oncogene
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Journal Article
·
Tue Feb 28 23:00:00 EST 1989
· J. Virol.; (United States)
·
OSTI ID:5814845
Reduced induction of c-fos but not of c-myc expressions in a nontumorigenic revertant R1 of EJ-ras-transformed NIH/3T3 cells treated with 12-O-tetradecanoylphorbol-13-acetate (TPA)
Journal Article
·
Sun Oct 01 00:00:00 EDT 1989
· Experimental Cell Research; (United States)
·
OSTI ID:5714492
Resistance to oncogenic transformation in revertant R1 of human ras-transformed NIH 3T3 cells
Journal Article
·
Mon May 01 00:00:00 EDT 1989
· Mol. Cell. Biol.; (United States)
·
OSTI ID:5703338
Related Subjects
550600* -- Medicine
560300 -- Chemicals Metabolism & Toxicology
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
CARCINOGENS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DNA SEQUENCING
ENZYMES
ESTERS
FIBROBLASTS
GENES
MAMMALS
MICE
MOLECULAR BIOLOGY
NUCLEOPROTEINS
ONCOGENES
ORGANIC COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PROMOTERS
PROTEINS
RODENTS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION FACTORS
TRANSFERASES
TUMOR PROMOTERS
VERTEBRATES
560300 -- Chemicals Metabolism & Toxicology
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
CARCINOGENS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DNA SEQUENCING
ENZYMES
ESTERS
FIBROBLASTS
GENES
MAMMALS
MICE
MOLECULAR BIOLOGY
NUCLEOPROTEINS
ONCOGENES
ORGANIC COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PROMOTERS
PROTEINS
RODENTS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION FACTORS
TRANSFERASES
TUMOR PROMOTERS
VERTEBRATES