Reduced induction of c-fos but not of c-myc expressions in a nontumorigenic revertant R1 of EJ-ras-transformed NIH/3T3 cells treated with 12-O-tetradecanoylphorbol-13-acetate (TPA)

Journal Article · Sun Oct 01 04:00:00 EDT 1989 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(89)90350-9· OSTI ID:5714492

Suzuki, Hiroaki; Fujita, Hisakazu; Ogiso, Yoshifumi; Oda, Atsushi; Kuzumaki, Noboru; Uchino, Junichi ^[1]

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1. Hokkaido Univ., Sapporo (Japan)

It has been reported that both c-fos and c-myc mRNAs are induced in NIH/3T3 cells after 12-O-tetradecanoylphorbol-13-acetate (TPA) treatment. The authors have studied the effect of TPA on the expression of c-fos and c-myc in EJ-ras-transformed NIH/3T3 and its nontumorigenic flat revertant R1 cells. Although TPA treatment induces c-myc mRNA, as in the case of NIH/3T3 cells, the induced level of c-fos mRNA is greatly reduced not only in slow growing EJ-ras-transformed NIH/3T3 but also in quiescent R1 cells. In addition, serum-induced c-fos expression is also reduced in EJ-ras-transformed NIH/3T3 and R1 cells. These observations suggest that the pathway from TPA to c-fos gene is different from that to c-myc gene and that the former pathway is down-regulated in association not with the transformed phenotype, but with EJ-ras expression, and it is possible that this reduced induction of c-fox is not specific to TPA.

• OSTI ID: 5714492
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 184:2; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
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BIOLOGICAL PATHWAYS
CARCINOGENESIS
CARCINOGENS
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
ESTERS
FIBROBLASTS
GENE REGULATION
GENES
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ONCOGENES
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PHORBOL ESTERS
RNA
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