12-O-tetradecanoylphorbol-13-acetate disrupts actin filaments and focal contacts and enhances binding of fibronectin-coated latex beads to 3T3-L1 cells
- Hiroshima Univ. School of Dentistry (Japan)
The effect of a tumor-promoting phorbol ester on the binding of fibronectin-coated beads to 3T3-L1 cells was studied to clarify the relationship between the binding of fibronectin to the cells, cell adhesion, and the organization of actin filaments. Interference-reflection microscopy revealed focal contacts of 3T3-L1 cells with the substratum. Stress fibers observed after rhodomine-phalloidin staining were well-developed in the cells. Treatment of the cells for 20 min with 12-O-tetradecanoylphorbol-13-acetate (TPA), but not with phorbol, disrupted focal contacts and caused a reorganization of stress fibers to generate actin ribbons. Treatment of the cells with TPA enhanced the binding of beads coated with human plasma fibronectin to the cells, as observed after incubation for 6 h with the beads. The TPA-induced increase in the percentage of cells with bound beads was dependent on the duration of treatment with TPA and on the concentration of TPA. Treatment of the cells with TPA also enhanced proliferation of cells in a dose-dependent manner. Furthermore, treatment of the cells with phorbol did not enhance the binding of beads coated with fibronectin. These results suggest that TPA specifically enhances the binding of fibronectin-coated beads to 3T3-L1 cells, and that TPA-induced binding of the beads may be related to disruption of focal contacts and reorganization of actin filaments.
- OSTI ID:
- 5447560
- Journal Information:
- Experimental Cell Research; (United States), Vol. 178:2; ISSN 0014-4827
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
FIBROBLASTS
ADHESION
PHORBOL ESTERS
BIOLOGICAL EFFECTS
SCLEROPROTEINS
BIOCHEMISTRY
ACTIN
COAGULANTS
DOSE-RESPONSE RELATIONSHIPS
RECEPTORS
SCANNING ELECTRON MICROSCOPY
ANIMAL CELLS
CARCINOGENS
CHEMISTRY
CONNECTIVE TISSUE CELLS
DRUGS
ELECTRON MICROSCOPY
ESTERS
HEMATOLOGIC AGENTS
MEMBRANE PROTEINS
MICROSCOPY
ORGANIC COMPOUNDS
PROTEINS
SOMATIC CELLS
560300* - Chemicals Metabolism & Toxicology