T-antigen-independent replication of polyomavirus DNA in murine embryonal carcinoma cells
Journal Article
·
· Mol. Cell. Biol.; (United States)
Expression of wild-type polyomavirus (Py) is restricted in murine embryonal carcinoma (EC) cells. The block appears to be located at the level of early transcription. Since no T antigen is produced, the authors investigated the fate of viral DNA upon infection of these cells; they showed that wild-type Py DNA replicates efficiently in all EC cells, probably via a T-antigen-independent mechanism. Furthermore, they studied, at permissive and restrictive temperatures, the replication of tsa (thermosensitive for T antigen) viral DNA of an in vitro-constructed deletion mutant lacking part of the early region coding sequences and of a double mutant carrying both the tsa mutation and the PyEC F9 mutation (allowing expression of early and late viral functions in EC cells). The results imply that replication of wild-type A2 strain Py DNA can occur in EC cells in the absence of a functional T antigen. However, this protein clearly enhances viral DNA replication and is absolutely required in differentiated cells.
- Research Organization:
- Laboratoire des Virus Oncogenes de l'Universite Pierre et Marie Curie, Institut de Recherches Scientifiques sur le Cancer, 94802 Villejuif Cedex
- OSTI ID:
- 5942141
- Journal Information:
- Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 4:2; ISSN MCEBD
- Country of Publication:
- United States
- Language:
- English
Similar Records
Small and middle T antigens contribute to lytic and abortive polyomavirus infection
Host range and cell cycle activation properties of polyomavirus large T-antigen mutants defective in pRB binding
Functional analysis of the individual enhancer core sequences of polyomavirus: Cell-specific uncoupling of DNA replication from transcription
Journal Article
·
Thu Jan 31 23:00:00 EST 1985
· J. Virol.; (United States)
·
OSTI ID:5304329
Host range and cell cycle activation properties of polyomavirus large T-antigen mutants defective in pRB binding
Journal Article
·
Mon Oct 31 23:00:00 EST 1994
· Journal of Virology
·
OSTI ID:153480
Functional analysis of the individual enhancer core sequences of polyomavirus: Cell-specific uncoupling of DNA replication from transcription
Journal Article
·
Sun May 01 00:00:00 EDT 1988
· Mol. Cell. Biol.; (United States)
·
OSTI ID:7027022
Related Subjects
550400* -- Genetics
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIGENS
BIOSYNTHESIS
CARCINOMAS
CELL CULTURES
CELL CYCLE
CELL PROLIFERATION
CHROMOSOME LOSSES
DISEASES
DNA
DNA REPLICATION
EMBRYONIC CELLS
GENE AMPLIFICATION
GENE MUTATIONS
GENE REPRESSORS
IN VITRO
INFECTIOUS DISEASES
LOSSES
METABOLISM
MICROORGANISMS
MOLECULAR BIOLOGY
MUTATIONS
NEOPLASMS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOPROTEINS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHENOTYPE
POLYOMA VIRUS
PROTEINS
SYNTHESIS
TEMPERATURE EFFECTS
TRANSCRIPTION
VIRAL DISEASES
VIRUSES
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIGENS
BIOSYNTHESIS
CARCINOMAS
CELL CULTURES
CELL CYCLE
CELL PROLIFERATION
CHROMOSOME LOSSES
DISEASES
DNA
DNA REPLICATION
EMBRYONIC CELLS
GENE AMPLIFICATION
GENE MUTATIONS
GENE REPRESSORS
IN VITRO
INFECTIOUS DISEASES
LOSSES
METABOLISM
MICROORGANISMS
MOLECULAR BIOLOGY
MUTATIONS
NEOPLASMS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOPROTEINS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHENOTYPE
POLYOMA VIRUS
PROTEINS
SYNTHESIS
TEMPERATURE EFFECTS
TRANSCRIPTION
VIRAL DISEASES
VIRUSES