Small and middle T antigens contribute to lytic and abortive polyomavirus infection

Journal Article · Thu Jan 31 23:00:00 EST 1985 · J. Virol.; (United States)

OSTI ID:5304329

Tuerler, H; Salomon, C

Using three different polyomavirus hr-t mutants and two polyomavirus mlT mutants, the authors studied induction of S-phase by mutants and wild-type virus in quiescent mouse kidney cells, mouse 3T6 cells, and FR 3T3 cells. At different times after infection, they measured the proportion of T-antigen-positive cells, the incorporation of (/sup 3/H)thymidine, the proportion of DNA-synthesizing cells, and the increase in total DNA, RNA, and protein content of the cultures. In permissive mouse cells, they also determined the amount of viral DNA and the proportion of viral capsid-producing cells. In polyomavirus hr-t mutant-infected cultures, the onset of host DNA replication was delayed by several hours, and a smaller proportion of T-antigen-positive cells entered S-phase than in wild-type-infected cultures. Of the two polyomavirus mlT mutants studied, dl-23 behaved similarly to wild-type virus in many, but not all, parameters tested. The poorly replicating but well-transforming mutant dl-8 was able to induce S-phase, and (in permissive cells) progeny virus production, in only about one-third of the T-antigen-positive cells. From the experiments, the authors concluded that mutations affecting small and middle T-antigen cause a reduction in the proportion of cells responding to virus infection and a prolongation of the early phase, i.e., the period before cells center S-phase. In hr-t mutant-infected mouse 3T6 cells, production of viral DNA was <10% of that in wild-type-infected cultures; low hr-t progeny production in 3T6 cells was therefore largely due to poor viral DNA replication.

• Research Organization: Univ. of Geneva, Switzerland
• OSTI ID: 5304329
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 53:2; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIGENS
AZINES
BODY
CELL CULTURES
DISEASES
DNA
DNA REPLICATION
HETEROCYCLIC COMPOUNDS
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
KIDNEYS
LABELLED COMPOUNDS
MAMMALS
MICE
MICROORGANISMS
MITOGENS
MUTANTS
MUTATIONS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PARASITES
PROTEINS
PYRIMIDINES
RIBOSIDES
RNA
RODENTS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
VIRAL DISEASES
VIRUSES