Regulation of c-myc and c-fos mRNA levels by polyomavirus: distinct roles for the capsid protein VP/sub 1/ and the viral early proteins

Journal Article · Sun Mar 01 04:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.5.1210· OSTI ID:6372513

Zullo, J; Stiles, C D; Garcea, R L

The levels of c-myc, c-fos, and JE mRNAs accumulate in a biphasic pattern following infection of quiescent BALB/c 3T3 mouse cells with polyomavirus. Maximal levels of c-myc and c-fos mRNAs were seen within 1 hr and were nearly undetectable at 6 hr after infection. At 12 hr after infection mRNA levels were again maximal and remained elevated thereafter. Empty virions (capsids) and recombinant VP/sub 1/ protein, purified from Escherichia coli, induced the early but not the late phase of mRNA accumulation. Virions, capsids, and recombinant VP/sub 1/ protein stimulated (/sup 3/H)thymidine nuclear labeling and c-myc mRNA accumulation in a dose-responsive manner paralleling their affinity for the cell receptor for polyoma. The second phase of mRNA accumulation is regulated by the viral early gene products, as shown by polyomavirus early gene mutants and by a transfected cell line (336a) expressing middle tumor antigen upon glucocorticoid addition. These results suggest that polyomavirus interacts with the cell membrane at the onset of infection to increase the levels of mRNA for the cellular genes associated with cell competence for DNA replication, and subsequently these levels are maintained by the action of the early viral proteins.

• Research Organization: Harvard Medical School, Boston, MA
• OSTI ID: 6372513
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:5; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AZINES
CELL CONSTITUENTS
CELL CYCLE
CELL MEMBRANES
CONFIGURATION INTERACTION
CONNECTIVE TISSUE CELLS
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
FIBROBLASTS
GENE REGULATION
HETEROCYCLIC COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
MESSENGER-RNA
MICE
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
POLYOMA VIRUS
PROTEINS
PYRIMIDINES
RIBOSIDES
RNA
RODENTS
SOMATIC CELLS
THYMIDINE
TRITIUM COMPOUNDS
VERTEBRATES
VIRUSES