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Cooperation between the polyomavirus Middle-T-antigen gene and the human c-myc oncogene in a rat thyroid epithelial differentiated cell line: Model of in vitro progression

Journal Article · · Mol. Cell. Biol.; (United States)
DOI:https://doi.org/10.1128/MCB.8.5.2261· OSTI ID:6902970
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Two rat thyroid epithelial differentiated cell lines, PC CI 3 and PC myc, were infected with the polyoma murine leukemia virus (PyMLV) carrying the Middle-T-antigen gene of polyomavirus. After infection, both cell lines acquired the typical markers of neoplastic transformation; however, the PC myc cells showed a greater malignant phenotype. Furthermore, the thyroid differentiated functions were completely suppressed in PC myc cells transformed by PyMLV, whereas they were, at least partially, retained in PC CI 3 cells transformed by PyMLV, and in particular, thyroglobulin synthesis and secretion were not affected at all. Since no differences in the expression of the middle-T-antigen gene were observed in the two PyMLV-transformed cell lines, the different properties shown by these two infected cell lines must be ascribed to the expression of the c-myc oncogene.

Research Organization:
Centro di Endocrinologia ed Oncologia Sperimentale C.N.R., Dipartimento di Biologia e Patologia Cellulare e Molecolare, II Facolta di Medicina e Chirurgia, Universita di Napoli, via Pansini 5, 80131 Naples (IT)
OSTI ID:
6902970
Journal Information:
Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:5; ISSN MCEBD
Country of Publication:
United States
Language:
English

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Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIGENS
BIOLOGICAL MATERIALS
BIOLOGICAL MODELS
BIOLOGICAL PATHWAYS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CELL DIFFERENTIATION
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
EPITHELIUM
GENE REPRESSORS
GENES
GENETIC MAPPING
IN VITRO
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAPPING
MATERIALS
MICROORGANISMS
MOLECULAR BIOLOGY
NUCLEOPROTEINS
ONCOGENES
ONCOGENIC TRANSFORMATIONS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
POLYOMA VIRUS
PROTEINS
RATS
RODENTS
SOMATIC CELLS
THYROID CELLS
TISSUES
VERTEBRATES
VIRUSES