Hydroxyproline in the major capsid protein VP1 of polyomavirus
Amino acid analysis of (/sup 3/H)proline-labeled polyomavirus major capsid protein VP1 by two-dimensional paper chromatography of the acid-hydrolyzed protein revealed the presence of /sup 3/H-labeled hydroxyproline. Addition of the proline analog L-azetidine-2-carboxylic acid to infected mouse kidney cell cultures prevented or greatly reduced hydroxylation of proline in VP1. Immunofluorescence analysis performed on infected cells over a time course of analog addition revealed that virus proteins were synthesized but that transport from the cytoplasm to the nucleus was impeded. A reduction in the assembly of progeny virions demonstrated by CsCl gradient purification of virus from (/sup 35/S)methionine-labeled infected cell cultures was found to correlate with the time of analog addition. These results suggest that incorporation of this proline analog into VP1, accompanied by reduction of the hydroxyproline content of the protein, influences the amount of virus progeny produced by affecting transport of VP1 to the cell nucleus for assembly into virus particles.
- Research Organization:
- Kansas State Univ., Manhattan (USA)
- OSTI ID:
- 6156449
- Journal Information:
- J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 63:6; ISSN JOVIA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
FLUORESCENCE
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LIFE CYCLE
LIGHT NUCLEI
LIPOTROPIC FACTORS
LUMINESCENCE
MAMMALS
MEMBRANE TRANSPORT
METABOLISM
METHIONINE
MICE
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PARASITES
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
RADIOISOTOPES
RODENTS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TWO-DIMENSIONAL ELECTROPHORESIS
VERTEBRATES
VIRAL DISEASES
VIRUSES