Herpes simplex virus type 1 tegument proteins VP1/2 and UL37 are associated with intranuclear capsids

Journal Article · Thu May 10 04:00:00 EDT 2007 · Virology

DOI:https://doi.org/10.1016/j.virol.2006.11.031· OSTI ID:20977020

Bucks, Michelle A ^[1]; O'Regan, Kevin J ^[1]; Murphy, Michael A ^[1]; Wills, John W ^[1]; Courtney, Richard J ^[1]

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1. Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033 (United States)

The assembly of the tegument of herpes simplex virus type 1 (HSV-1) is a complex process that involves a number of events at various sites within virus-infected cells. Our studies focused on determining whether tegument proteins, VP1/2 and UL37, are added to capsids located within the nucleus. Capsids were isolated from the nuclear fraction of HSV-1-infected cells and purified by rate-zonal centrifugation to separate B capsids (containing the scaffold proteins and no viral DNA) and C capsids (containing DNA and no scaffold proteins). Western blot analyses of these capsids indicated that VP1/2 associated primarily with C capsids and UL37 associated with B and C capsids. The results demonstrate that at least two of the tegument proteins of HSV-1 are associated with capsids isolated from the nuclear fraction, and these capsid-tegument protein interactions may represent initial events of the tegumentation process.

• OSTI ID: 20977020
• Journal Information: Virology, Journal Name: Virology Journal Issue: 2 Vol. 361; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL NUCLEI
CENTRIFUGATION
DNA
HERPES SIMPLEX
PROTEINS
VIRUSES