Verification of the production of peptide leukotrienes (LT) in traumatic shock

Conference · Tue Mar 04 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7023606

Hock, C E; Craft, D V; Lefer, D J; Lefer, A M

Both lipoxygenase inhibition and leukotriene receptor antagonism have been demonstrated to provide significant protection in traumatic shock. Despite these findings, leukotrienes have not been found in circulating blood in Noble-Collip drum induced traumatic shock using radioimmunoassay techniques. Anesthetized rats subjected to Noble-Collip drum trauma developed a shock state characterized by a significant reduction in mean arterial blood pressure, a 4.5 fold increase in plasma cathepsin D activity, a 3-fold increase in myocardial depressant factor activity and a mean survival time of 1.9 +/- 0.3 hours. Plasma and bile samples were analyzed by reverse phase high pressure liquid chromatography to determine LT production in this shock model. No detectable peptide leukotrienes or their metabolites were found in plasma. The major peptide leukotriene from bile eluted between LTC/sub 4/ and LTD/sub 4/ and corresponds to a metabolite of LTE/sub 4/, N-acetyl-LTE/sub 4/. This metabolite increased from 6 +/- 3 to 41 +/- 4 units in traumatic shock when compared to sham trauma (p < 0.01). Infusion of exogenous LTC/sub 4/, LTD/sub 4/ and LTE/sub 4/ (10 ..mu..g/kg/h) also resulted in the metabolism of > 90% of the parent LT to this metabolite in bile. Therefore, plasma LTs accumulate in the bile following trauma in rats. Moreover, LTC/sub 4/, LTD/sub 4/ and LTE/sub 4/ apparently are rapidly metabolized to N-acetyl LTE/sub 4/. These findings further support a role for leukotrienes in the pathogenesis of traumatic shock in rats.

• Research Organization: Thomas Jefferson Univ., Philadelphia, PA
• OSTI ID: 7023606
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARACHIDONIC ACID
BILE
BIOLOGICAL MATERIALS
BIOLOGICAL SHOCK
BIOSYNTHESIS
BLOOD PRESSURE
BODY FLUIDS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MATERIALS
METABOLITES
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PATHOGENESIS
PATHOLOGICAL CHANGES
PEPTIDES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RATS
RODENTS
SEPARATION PROCESSES
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES