Radioimmunoassay for leukotriene D/sub 4/ leukotriene E/sub 4/

Conference · Fri May 01 00:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6173145

Kozlowski, A; Smith, J B

A conjugate of leukotriene (LT) D/sub 4/ and keyhole limpet hemocyanin was made using difluorodinitrobenzene as link to the free amine on LTD/sub 4/. This conjugate was emulsified in Freund's adjuvant and injected intradermally into New Zealand white rabbits at two week intervals. Using charcoal to separate bound from free and /sup 3/H-LTD/sub 4/ as the ligand, the antiserum obtained after 6 months displays 100% cross-reactivity between LTD/sub 4/ and LTE/sub 4/, 5.3% with LTC/sub 4/ and negligible affinities for other arachidonic acid metabolites or glutathione. The /sup 3/H-LTD/sub 4/ is displaced by 50% with 75pg of LTD/sub 4/ or LTE/sub 4/ and the detection limit of this assay is 12.5pg. Since LTE/sub 4/ is the major leukotriene metabolite present in human urine, this RIA may be useful in determining the daily excretion of leukotrienes.

• Research Organization: Temple Univ. School of Medicine, Philadelphia, PA
• OSTI ID: 6173145
• Report Number(s): CONF-870644-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 46:6; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMALS
ARACHIDONIC ACID
CARBOXYLIC ACIDS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIGANDS
MAMMALS
METABOLITES
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES