Significance of production of peptide leukotrienes in murine traumatic shock

Journal Article · Tue Jul 01 04:00:00 EDT 1986 · Am. J. Physiol.; (United States)

OSTI ID:6523539

Craft, D V; Lefer, D J; Hock, C E; Lefer, A M

The authors studied the formation of a leukotriene metabolite in plasma and bile during traumatic shock. Anesthetized rats subjected to Noble-Collip drum trauma developed a lethal shock state characterized by a survival time of 1.9 +/- 0.3h, a 4.5-fold increase in plasma cathepsin D activity, and a reduction in mean arterial blood pressure to 45 +/- 2 mmHg compared with 108 +/- 5 mmHg in sham-shock controls. Plasma and bile samples were analyzed by reverse-phase high-pressure liquid chromatography (HPLC) for peptide leukotrienes, and their retention times were confirmed by co-elution with radioactive standards, radioimmunoassay (RIA), and UV spectrophotometry. No leukotrienes or metabolites were found in plasma. The major peptide leukotriene from bile was eluted between LTC/sub 4/ and LTD/sub 4/ and corresponds to a metabolite of LTE/sub 4/, N-acetyl-LTE/sub 4/, which is also produced during endotoxin shock. The metabolite increased nearly sevenfold in traumatic shock compared with sham trauma. The identity of the metabolite was confirmed by UV scan, which revealed a spectrum consistent with a peptide leukotriene and similar to that of previously reported spectra for N-acetyl-LTE/sub 4/. In conclusion, peptide leukotrienes are rapidly cleared from the blood and appear in the bile as N-acetyl-LTE/sub 4/, a metabolite of the peptide leukotrienes. These findings support a role of the peptide leukotrienes in the pathogenesis of traumatic shock.

• Research Organization: Thomas Jefferson Univ., Philadelphia, PA
• OSTI ID: 6523539
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 251:1; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BILE
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL SHOCK
BLOOD
BLOOD PLASMA
BLOOD PRESSURE
BLOOD-PLASMA CLEARANCE
BODY FLUIDS
CHROMATOGRAPHY
CLEARANCE
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LIPIDS
LIPOPROTEINS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MATERIALS
METABOLITES
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RATS
RODENTS
SEPARATION PROCESSES
SPECTROPHOTOMETRY
TRACER TECHNIQUES
VERTEBRATES